Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.
Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.
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DOI：
10.1530/rep-20-0494
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发表时间：
2021-04
期刊：
影响因子：
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通讯作者：
Carnevale EM
中科院分区：
文献类型：
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作者：
Catandi GD;Obeidat YM;Broeckling CD;Chen TW;Chicco AJ;Carnevale EM
高龄产妇与生育力和卵母细胞质量下降有关。我们使用新的代谢微传感器来评估母马年龄对单个卵母细胞和胚胎代谢功能的影响，这在哺乳动物中还没有得到类似的研究。我们假设马母马衰老影响卵母细胞和体外产生的早期胚胎的代谢功能、卵母细胞线粒体DNA(MtDNA)拷贝数以及参与能量代谢的代谢物在卵母细胞和卵丘细胞中的相对丰度。样本来自年轻(≤14岁)和老年(≥20岁)母马的排卵前卵泡。液、气相色谱-质联用分析了母马卵母细胞及其卵丘细胞中代谢产物的相对丰度，结果表明，老年母马卵母细胞中游离脂肪酸含量较低，而卵丘细胞中游离脂肪酸含量较高。在含有氧气和pH微型传感器的微室中，有氧代谢和无氧代谢的定量(分别测量为耗氧率(OCR)和胞外酸化率(ECAR))表明，与年轻母马相比，来自老年卵母细胞的卵母细胞和第二天胚胎的代谢功能和能力降低。在成熟卵母细胞中，线粒体DNA被实时定量，在不同年龄组之间没有差异，也不能反映线粒体的功能。来自年轻母马的注入精子的卵母细胞比来自老年母马的显著更多的卵子导致了囊胚。我们的结果表明，卵母细胞和胚胎代谢活动的下降可能导致老年女性发育能力和生育能力的下降。
Advanced maternal age is associated with a decline in fertility and oocyte quality. We used novel metabolic microsensors to assess effects of mare age on single oocyte and embryo metabolic function, which has not yet been similarly investigated in mammalian species. We hypothesized that equine maternal aging affects the metabolic function of oocytes and in vitro-produced early embryos, oocyte mitochondrial DNA (mtDNA) copy number, and relative abundance of metabolites involved in energy metabolism in oocytes and cumulus cells. Samples were collected from preovulatory follicles from young (≤14 years) and old (≥20 years) mares. Relative abundance of metabolites in metaphase II oocytes (MII) and their respective cumulus cells, detected by liquid and gas chromatography coupled to mass spectrometry, revealed that free fatty acids were less abundant in oocytes and more abundant in cumulus cells from old versus young mares. Quantification of aerobic and anaerobic metabolism, respectively measured as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in a microchamber containing oxygen and pH microsensors, demonstrated reduced metabolic function and capacity in oocytes and day-2 embryos originating from oocytes of old when compared to young mares. In mature oocytes, mtDNA was quantified by real-time PCR, was not different between the age groups and not indicative of mitochondrial function. Significantly more sperm-injected oocytes from young than old mares resulted in blastocysts. Our results demonstrate a decline in oocyte and embryo metabolic activity that potentially contributes to the impaired developmental competence and fertility in aged females.