Distribution of cellulosic wall in the anthers of Arabidopsis during microsporogenesis
Distribution of cellulosic wall in the anthers of Arabidopsis during microsporogenesis
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DOI：
10.1007/s00299-013-1487-1
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发表时间：
2013-11-01
影响因子：
6.2
通讯作者：
Tsutsumi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Matsuo, Yuichi;Arimura, Shin-ichi;Tsutsumi, Nobuhiro
纤维素特异性染色显示绒毡层细胞和小孢子母细胞在减数分裂开始前失去纤维素壁。小孢子母细胞中纤维素壁的降解可能不依赖于绒毡层细胞（或TPD 1）。在这里，我们报告说，绒毡层细胞的花药拟南芥似乎没有一个纤维素壁的基础上染色Calcofluor和文艺复兴2200。在造孢细胞形成过程中，花药组织中均存在纤维素壁。在减数分裂前，绒毡层细胞和小孢子母细胞上几乎不存在。在一个spl/nzz突变体中，它缺乏几个组成部分（小孢子母细胞，绒毡层，中层和药室内壁），纤维素壁在所有花药细胞中检测到。在另一个突变体中，绒毡层决定子1（tpd 1），缺乏绒毡层，有更多的小孢子母细胞，纤维素壁几乎没有减数分裂前的小孢子母细胞，类似于野生型。这些结果表明，绒毡层细胞和小孢子母细胞在小孢子母细胞形成过程中失去纤维素壁，并且细胞壁降解发生在SPL/NZZ下游，并且不依赖于TPD 1。
Cellulose-specific staining revealed that tapetal cells and microsporocytes lose cellulosic walls before the onset of meiosis. Cellulosic wall degradation in microsporocytes might be independent of tapetal cells (or TPD1).Some cell types in a variety of angiosperms have been reported to lack cell walls. Here, we report that the tapetal cells of the anther of Arabidopsis thaliana did not appear to have a cellulosic wall based on staining with Calcofluor and Renaissance 2200. During sporogenous cell formation, cellulosic wall was present in all anther tissues. However, before meiosis it was almost absent on the tapetal cells and on the microsporocytes. In a sporocyteless/nozzle (spl/nzz) mutant, which lacks several components (microsporocytes, tapetum, middle layer and endothecium), cellulosic wall was detected in all anther cells. In another mutant, tapetum determinant1 (tpd1), which lacks tapetum and has more microsporocytes, cellulosic wall was almost absent on the microsporocytes before meiosis, similar to the wild type. These results suggest that the tapetum cells and microsporocytes lose cellulosic walls during microsporocyte formation, and that cell wall degradation occurs downstream of SPL/NZZ and is independent of TPD1.