T-1-Mediated Nanosensor for Immunoassay Based on an Activatable MnO2 Nanoassembly
T-1-Mediated Nanosensor for Immunoassay Based on an Activatable MnO2 Nanoassembly
复制标题
基于可激活 MnO2 纳米组件的 T-1 介导的免疫测定纳米传感器
DOI：
10.1021/acs.analchem.7b04817
复制
发表时间：
2018
影响因子：
7.4
通讯作者：
Jiang Xingyu
中科院分区：
文献类型：
--
作者：
Liu Zixin;Xianyu Yunlei;Zheng Wenshu;Zhang Jiangjiang;Luo Yunjing;Chen Yiping;Dong Mingling;Wu Jing;Jiang Xingyu
目前用于检测复杂样品中的痕量目标的磁弛豫开关（MRS）传感器仍然在相对低的灵敏度和差的稳定性方面受到限制。为了应对这一挑战，我们开发了一种基于纵向弛豫时间（T1）的纳米传感器，该传感器利用MnO2纳米组装体还原时释放的Mn 2+诱导T1的变化，从而可以大大提高灵敏度并克服传统MRS的“钩效应”。通过抗原与抗体功能化的MnO2纳米组装体之间的特异性相互作用，从纳米组装体释放的Mn2+的T1信号由抗原定量测定，这允许对靶进行高度灵敏和直接的检测。这种方法拓宽了磁性生物传感器的适用范围，在疾病生物标志物的早期诊断中具有很大的应用潜力。
Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T1)-based nanosensor by using Mn2+released from the reduction of a MnO2nanoassembly that can induce the change of T1, and thus can greatly improve the sensitivity and overcome the “hook effect” of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO2nanoassembly, the T1signal of Mn2+released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.