Targeting frequency for deletion vectors in embryonic stem cells
Targeting frequency for deletion vectors in embryonic stem cells
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胚胎干细胞中缺失载体的靶向频率
DOI：
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发表时间：
1994
影响因子：
5.3
通讯作者：
Allan Bradley
中科院分区：
文献类型：
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作者：
Hongbing Zhang;Paul Hasty;Allan Bradley
我们分析了一系列针对小鼠胚胎干细胞中次黄嘌呤磷酸核糖基转移酶（hprt）位点的替代缺失载体所产生的基因靶向频率和重组产物。我们发现19.2 kb缺失的靶向频率与3 kb缺失或插入1.7 kb片段的传统替换事件相当。我们还观察到这些缺失向量的不同整合模式。这一发现的结果是，胚胎干细胞中广泛的基因组缺失是可行的。
We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.
次黄嘌呤磷酸核糖转移酶基因的结构、表达和突变。
DOI：
10.1073/pnas.81.7.2147
发表时间：
1984
影响因子：
11.1
作者：
Melton,DW;Konecki,DS;Brennand,J;Caskey,CT
通讯作者：
Caskey,CT