Probing cell structure by controlling the mechanical environment with cell-substrate interactions
Probing cell structure by controlling the mechanical environment with cell-substrate interactions
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DOI：
10.1016/j.jbiomech.2008.10.014
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发表时间：
2009-01-19
影响因子：
2.4
通讯作者：
LeDuc, Philip R.
中科院分区：
文献类型：
--
作者：
Cheng, Chao-Min;Steward, Robert L., Jr.;LeDuc, Philip R.
最近的研究结果表明，细胞形态和结构对机械刺激的敏感性非常高。机械刺激通常与细胞-基质相互作用结合，从而影响分子反应并决定细胞命运，包括细胞凋亡、增殖和分化。为了理解这些效应，因为它们具体涉及压缩机械刺激和地形控制，我们开发了一种微加工系统，以在聚二甲基硅氧烷（PDMS）微通道表面上生长细胞，在该微通道表面上我们保持压缩刺激。我们还探测了对不同刚度的PDMS基底进行压缩机械刺激后的细胞反应。在这些情况下，我们检查了在施加局部压缩刺激后附着于我们的基底的活细胞中的细胞骨架和形态学变化。我们发现，整体形态和细胞结构，包括肌动蛋白细胞骨架，定向的方向上的压缩应变施加和沿着地形微通道。此外，通过比较材料刚度的地形响应，我们发现在微通道上培养的细胞的细胞面积与较软的PDMS相比增加了40%，并且当使用较软的PDMS时，细胞面积比未改性的PDMS减少了30%。这些发现对包括细胞-材料相互作用、机械传导和组织工程在内的多种领域的研究具有启示意义。(C)2008爱思唯尔有限公司版权所有。
Recent results demonstrate the exquisite sensitivity of cell morphology and structure to mechanical Stimulation. Mechanical stimulation is often coupled with cell-substrate interactions that can, in turn, influence molecular response and determine cellular fates including apoptosis, proliferation, and differentiation. To understand these effects as they specifically relate to compressive mechanical stimulation and topographic control, we developed a microfabricated system to grow cells on polydimethylsiloxane (PDMS) rnicrochannel surfaces where we maintained compression stimulation. We also probed cellular response following compressive mechanical Stimulation to PDMS substrates of varying stiffness. In these instances, we examined cytoskeletal and morphologic changes in living cells attached to Our substrate following the application of localized compressive Stimulation. We found that the overall morphology and cell Structure, including the actin cytoskeleton, oriented in the direction of the compressive strain applied and along the topographic microchannels. Furthermore by comparing topographic response to material stiffness, we found a 40% increase in cell area for cells Cultured on the microchannels versus softer PDMS as well as a decreased cell area of 30% when using softer PDMS over unmodified PDMS. These findings have implications for research in a diversity of fields including cell-material interactions, mechanotransduction, and tissue engineering. (C) 2008 Elsevier Ltd. All rights reserved.