Specific expression of secretogranin II in magnocellular vasopressin neurons of the rat supraoptic and paraventricular nucleus in response to osmotic stimulation
Specific expression of secretogranin II in magnocellular vasopressin neurons of the rat supraoptic and paraventricular nucleus in response to osmotic stimulation
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DOI：
10.1016/s0006-8993(97)00462-9
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发表时间：
1997-08-08
期刊：
影响因子：
2.9
通讯作者：
VanLeeuwen, FW
中科院分区：
文献类型：
--
作者：
Ang, CW;Dotman, CH;VanLeeuwen, FW
由于促分泌素 II 被认为是调节分泌途径的标志物，因此通过免疫细胞化学详细研究了其在盐负荷 Wistar 大鼠下丘脑-神经垂体系统中的分布。尽管在渗透压挑战后，加压素和催产素神经元都受到刺激，但促分泌素 II 仅在 Wistar 大鼠视上核和室旁核的加压素能大细胞神经元亚群中表达。只有在渗透压挑战和秋水仙碱治疗阻断轴突运输相结合后，Secretogranin II 才能确定地显现出来。当不进行这些预处理时，仅观察到位于室旁核和视上核内的大细胞神经元周围的点状纤维。催产素能大细胞神经元从未表现出任何分泌粒素 II 免疫反应性，甚至在哺乳期间和秋水仙碱治疗后也没有。这些发现表明，促分泌素 II 在加压素能神经元的分泌活动增强过程中具有重要的功能。 (C) 1997 Elsevier Science B.V.
As secretogranin II is considered to be a marker for the regulated secretory pathway, its distribution in the hypothalamo-neurohypophyseal system of salt-loaded Wistar rats was studied in detail by immunocytochemistry. Although after an osmotic challenge both vasopressin and oxytocin neurons are stimulated, secretogranin II was exclusively expressed in a subpopulation of vasopressinergic magnocellular neurons in the supraoptic and paraventricular nucleus of Wistar rats. Secretogranin II was only surely visualized after a combination of osmotic challenge and blockade of axonal transport by colchicine treatment. When these pre-treatments were not performed, only punctate fibers situated around the magnocellular neurons within the paraventricular and supraoptic nucleus were observed. Oxytocinergic magnocellular neurons never displayed any secretogranin II immunoreactivity, not even during lactation and after colchicine treatment. These findings suggest that secretogranin II is of functional importance during enhanced secretory activity within vasopressinergic neurons. (C) 1997 Elsevier Science B.V.