Real-Time Assessment of Ultrasound-Mediated Drug Delivery Using Fibered Confocal Fluorescence Microscopy
Real-Time Assessment of Ultrasound-Mediated Drug Delivery Using Fibered Confocal Fluorescence Microscopy
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DOI：
10.1007/s11307-012-0568-9
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发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Moonen, Chrit
中科院分区：
文献类型：
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作者：
Derieppe, Marc;Yudina, Anna;Moonen, Chrit
对于具有细胞内作用模式的弱渗透性化合物，跨质膜转运是药物递送的关键步骤。本研究的目的是利用光纤共聚焦荧光显微镜（FCFM）实时监测超声介导的细胞非渗透性模型药物摄取，设计了一个体外装置，该装置由联合收割机、单层肿瘤细胞和SonoVue微泡组成的细胞室和FCFM系统组成。用SYTOX绿色染料检测细胞对超声波的摄取，发现在超声波作用10 s后，大多数细胞的荧光信号增强。信号增强的平均速率常数k = 0.23 ± 0.04 min（-1），表明了FCFM实时监测超声介导的细胞内给药的可行性。该方法允许定量评估模型药物摄取，持有很大的希望，进一步的本地药物输送研究。
Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM).An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake.The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23 +/- 0.04 min(-1).Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.