Aedes aegypti uses RNA interference in defense against Sindbis virus infection.
Aedes aegypti uses RNA interference in defense against Sindbis virus infection.
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DOI：
10.1186/1471-2180-8-47
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发表时间：
2008-03-17
期刊：
影响因子：
4.2
通讯作者：
Foy BD
中科院分区：
文献类型：
--
作者：
Campbell CL;Keene KM;Brackney DE;Olson KE;Blair CD;Wilusz J;Foy BD
RNA干扰（RNAi）是一种重要的抗病毒防御机制。埃及伊蚊基因组编码 RNAi 成分直向同源物，然而，这种蚊子的大多数种群很容易被黄病毒和甲病毒感染，并随后传播。本研究的目标是使用 Ae。埃及伊蚊作为模型系统，确定蚊子的抗病毒 RNAi 途径如何与重组辛德比斯病毒（SINV；披膜病毒科，甲病毒属）相互作用。在向蚊子注射同源 Ago2、Dcr2 或 TSN mRNA 的 dsRNA 后，SINV (TR339-eGFP) (+) 链 RNA、感染性病毒滴度和感染率短暂增加。在非沉默蚊子中感染后 2 天和 7 天检测到 SINV RNA 衍生的小 RNA，为 RNAi 途径活性提供了重要的确认。使用感染动力学显着差异的两种不同的重组 SINV 病毒（MRE16-eGFP 和 TR339-eGFP）来描述中肠中载体/病毒的相互作用。我们展示了感染期间对 RNAi 成分转录物和蛋白质水平的病毒依赖性影响。感染期间监测中肠 Ago2、Dcr2 和 TSN 转录物水平表明，与血液喂养对照相比，中肠中只有 TSN 转录物显着增加。 Ago2 蛋白水平在非感染性血粉后立即耗尽，并在 SINV 感染期间以病毒依赖性方式发生变化。我们证明了 Ae 中 RNAi 成分的沉默。埃及伊蚊导致 SINV 复制短暂增加。此外，Ae.病毒特异性 siRNA 的产生表明，埃及伊蚊 RNAi 在 SINV 感染期间处于活跃状态。最后，RNAi 反应以病毒依赖性方式变化。这些数据定义了 Ae 中 RNAi 抗病毒防御的重要特征。埃及伊蚊。
RNA interference (RNAi) is an important anti-viral defense mechanism. The Aedes aegypti genome encodes RNAi component orthologs, however, most populations of this mosquito are readily infected by, and subsequently transmit flaviviruses and alphaviruses. The goal of this study was to use Ae. aegypti as a model system to determine how the mosquito's anti-viral RNAi pathway interacts with recombinant Sindbis virus (SINV; family Togaviridae, genus Alphavirus). SINV (TR339-eGFP) (+) strand RNA, infectious virus titers and infection rates transiently increased in mosquitoes following dsRNA injection to cognate Ago2, Dcr2, or TSN mRNAs. Detection of SINV RNA-derived small RNAs at 2 and 7 days post-infection in non-silenced mosquitoes provided important confirmation of RNAi pathway activity. Two different recombinant SINV viruses (MRE16-eGFP and TR339-eGFP) with significant differences in infection kinetics were used to delineate vector/virus interactions in the midgut. We show virus-dependent effects on RNAi component transcript and protein levels during infection. Monitoring midgut Ago2, Dcr2, and TSN transcript levels during infection revealed that only TSN transcripts were significantly increased in midguts over blood-fed controls. Ago2 protein levels were depleted immediately following a non-infectious bloodmeal and varied during SINV infection in a virus-dependent manner. We show that silencing RNAi components in Ae. aegypti results in transient increases in SINV replication. Furthermore, Ae. aegypti RNAi is active during SINV infection as indicated by production of virus-specific siRNAs. Lastly, the RNAi response varies in a virus-dependent manner. These data define important features of RNAi anti-viral defense in Ae. aegypti.
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影响因子：
14.9
作者：
Lawson, Daniel;Arensburger, Peter;Atkinson, Peter;Besansky, Nora J;Bruggner, Robert V;Butler, Ryan;Campbell, Kathryn S;Christophides, George K;Christley, Scott;Dialynas, Emmanuel;Emmert, David;Hammond, Martin;Hill, Catherine A;Kennedy, Ryan C;Lobo, Neil F;MacCallum, M Robert;Madey, Greg;Megy, Karine;Redmond, Seth;Russo, Susan;Severson, David W;Stinson, Eric O;Topalis, Pantelis;Zdobnov, Evgeny M;Birney, Ewan;Gelbart, William M;Kafatos, Fotis C;Louis, Christos;Collins, Frank H
通讯作者：
Collins, Frank H
DOI：
10.4269/ajtmh.1998.58.663
发表时间：
1998-05-01
影响因子：
3.3
作者：
Higgs, S;Rayner, JO;Blair, CD
通讯作者：
Blair, CD
影响因子：
5.4
作者：
Myles, KM;Pierro, DJ;Olson, KE
通讯作者：
Olson, KE
DOI：
10.1073/pnas.0406983101
发表时间：
2004-12-07
影响因子：
11.1
作者：
Keene, KM;Foy, BD;Olson, KE
通讯作者：
Olson, KE
影响因子：
5.4
作者：
Gaines, PJ;Olson, KE;Blair, CD
通讯作者：
Blair, CD