C-Fos immunohistochemical mapping of the audiogenic seizure network and tonotopic neuronal hyperexcitability in the inferior colliculus of the Frings mouse
C-Fos immunohistochemical mapping of the audiogenic seizure network and tonotopic neuronal hyperexcitability in the inferior colliculus of the Frings mouse
复制标题
DOI：
10.1016/j.eplepsyres.2004.06.007
复制
发表时间：
2004-11-01
影响因子：
2.2
通讯作者：
White, HS
中科院分区：
文献类型：
--
作者：
Klein, BD;Fu, YH;White, HS
Frings小鼠是听源性癫痫发作（AGS）易感性的模型。在Frings小鼠中负责AGS表型的遗传基因座被命名为单基因听源性耳聋易感（MASS 1）。MASS 1的独特之处在于它是仅有的两个与离子通道突变无关的癫痫发作位点之一。此外，Frings小鼠表现出强大的AGS表型，表现出对声音诱导的强直性伸展性癫痫发作的高度和长期易感性。本研究的目的是使用c-Fos免疫组织化学映射的大脑结构参与的弗林斯AGS和检查神经元的过度兴奋性下丘，大脑结构，被认为是网站的AGS启动。AGS映射显示，强烈的癫痫诱导的神经元激活主要限于脑干癫痫发作网络中涉及的结构，包括下丘的外核和背核，如在其他AGS啮齿动物中观察到的。声学诱导的c-Fos的表达在下丘中央核亚AGS阈值音刺激显示出更高水平的神经元激活的AGS敏感的Frings，DBA/2 J和噪声引发的C57 BL/6 J小鼠相比，抗AGS的C57 BL/6 J和CF 1小鼠。AGS敏感小鼠也表现出c-Fos免疫反应性，与AGS抗性小鼠相比，c-Fos免疫反应性更集中在下丘的tonotopic反应域内。此外，Frings小鼠显示出显着更大的tonotopic高反应性相比，其他AGS-sensitive小鼠。(C)2004 Elsevier B. V.保留所有权利。
The Frings mouse is a model of audiogenic seizure (AGS) susceptibility. The genetic locus responsible for the AGS phenotype in the Frings mouse has been named monogenic audiogenic seizure-susceptible (MASS1). MASS1 is unique in that it is one of only two identified seizure loci that are not associated with an ion channel mutation. Furthermore, Frings mice display a robust AGS phenotype demonstrating very high and prolonged susceptibility to sound-induced tonic extension seizures. The purpose of this investigation was to use c-Fos immunohistochemistry to map the brain structures involved in the Frings AGS and to examine neuronal hyperexcitability in the inferior colliculus, the brain structure that is recognized as the site of AGS initiation. AGS mapping revealed that intense seizure-induced neuronal activation was mostly limited to structures involved in a brainstem seizure network, including the external and dorsal nuclei of the inferior colliculus, as observed in other AGS rodents. Acoustically induced c-Fos expression in the central nucleus of the inferior colliculus to sub-AGS threshold tone stimulations displayed a greater level of neuronal activation in AGS-susceptible Frings, DBA/2J and noise-primed C57BL/6J mice compared to AGS-resistant C57BL/6J and CF1 mice. The AGS-susceptible mice also displayed c-Fos immunoreactivity that was more focused within the tonotopic response domain of the inferior colliculus compared to AGS-resistant mice. Furthermore, Frings mice displayed significantly greater tonotopic hyper-responsiveness compared to other AGS-susceptible mice. (C) 2004 Elsevier B.V. All rights reserved.