BK Polyomavirus Diversity After Hematopoietic Stem Cell Transplantation.
BK Polyomavirus Diversity After Hematopoietic Stem Cell Transplantation.
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造血干细胞移植后的 BK 多瘤病毒多样性。
DOI：
10.1093/infdis/jiad117
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Blackard,JasonT
中科院分区：
文献类型：
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作者：
Odegard,ElizabethA;Meeds,HeidiL;Kleiboeker,StevenB;Ziady,Assem;Sabulski,Anthony;Jodele,Sonata;Seif,AlixE;Davies,StellaM;Laskin,BenjaminL;Blackard,JasonT
BK多瘤病毒（BKPyV）感染在造血干细胞移植（HSCT）后很常见，并与出血性膀胱炎（HC）的发生有关。BKPyV在HC发病机制中的作用尚未得到充分表征。我们使用先前建立的儿科HSCT患者队列研究了BKPyV多样性对HC发展的影响。在HSCT后1个月，有147份尿液样本具有可定量的BKPyV; 137份（93.2%）使用我们的内部聚合酶链反应方法进行扩增，并进行下一代测序。Ia亚型最常见（61.3%），其次是Ib 1亚型（31.4%）。在第1个月，Ia亚型样本的中位病毒载量高于Ib 1亚型。在整个蛋白质编码区，鉴定了与HC相关的APOBEC诱导的突变和特征模式。这是HSCT患者单队列最大的测序研究，为未来的分析提供了大量的序列数据资源。
BK polyomavirus (BKPyV) infection is common after hematopoietic stem cell transplantation (HSCT) and is associated with the development of hemorrhagic cystitis (HC). The role that BKPyV plays in the pathogenesis of HC is not well characterized. We investigated the impact of BKPyV diversity on the development of HC using a previously established cohort of pediatric HSCT patients. There were 147 urine samples with quantifiable BKPyV at month 1 after HSCT; 137 (93.2%) were amplified using our in-house polymerase chain reaction approach and sent for next-generation sequencing. Subtype Ia was most frequent (61.3%), followed by subtype Ib1 (31.4%). The median viral load of subtype Ia samples was higher than for subtype Ib1 at month 1. Across the protein coding regions, APOBEC-induced mutations and signature patterns associated with HC were identified. This is the largest sequencing study of a single cohort of HSCT patients, providing a vast resource of sequence data for future analyses.