Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow
Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow
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DOI：
10.1039/c8ay01798k
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发表时间：
2019-01-07
影响因子：
3.1
通讯作者：
Mayerich, David
中科院分区：
文献类型：
--
作者：
Guo, Jiaming;Keller, Keely A.;Mayerich, David
在体内，微血管系统提供细胞生存和功能所需的氧气、营养物质和可溶性因子。微血管的高度曲折、密集和相互连接的三维(3D)结构确保细胞接收这些关键组件。在组织工程模型中复制微血管结构的能力可以提供一种方法来产生大量的结构以及先进的微生理系统。同样，在工程微血管系统中诱导真实流动的能力对于重述体内类似的流动和运输至关重要。先进的生物制造技术能够在水凝胶中产生三维仿生微流控网络，然而，由于不正确的边界条件，这些模型可能会在流动中表现出系统的畸变。为了克服这个问题，我们开发了一种自动生成合成增强通道的方法，该方法可以在三维微流体网络中诱导所需的流动特性。这些扩大的入口和出口实施适当的边界条件，以实现特定的流动特性，并创建对图像引导制造技术有用的三维输出，以创建仿生微血管网络。
In vivo, microvasculature provides oxygen, nutrients, and soluble factors necessary for cell survival and function. The highly tortuous, densely-packed, and interconnected three-dimensional (3D) architecture of microvasculature ensures that cells receive these crucial components. The ability to duplicate microvascular architecture in tissue-engineered models could provide a means to generate large-volume constructs as well as advanced microphysiological systems. Similarly, the ability to induce realistic flow in engineered microvasculature is crucial to recapitulating in vivo-like flow and transport. Advanced biofabrication techniques are capable of generating 3D, biomimetic microfluidic networks in hydrogels, however, these models can exhibit systemic aberrations in flow due to incorrect boundary conditions. To overcome this problem, we developed an automated method for generating synthetic augmented channels that induce the desired flow properties within three-dimensional microfluidic networks. These augmented inlets and outlets enforce the appropriate boundary conditions for achieving specified flow properties and create a three-dimensional output useful for image-guided fabrication techniques to create biomimetic microvascular networks.