Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species
Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species
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DOI：
10.1016/s0168-9452(00)00208-9
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发表时间：
2000-06-29
期刊：
影响因子：
5.2
通讯作者：
Henry, RJ
中科院分区：
文献类型：
--
作者：
Cordeiro, GM;Taylor, GO;Henry, RJ
栽培甘蔗品种（Saccharum spp）源自 S. spontaneum (2n = 40-128) 和 S. officinarum (2n = 60 或 80) 物种之间复杂的种间杂交。为了分析这个复杂的基因组，研究了微卫星重复作为甘蔗遗传标记的潜力，包括其丰度、变异性和检测多态性的能力。从甘蔗 cv Q124 构建的富集基因组 DNA 文库中鉴定出一组用于栽培甘蔗基因组分析的微卫星标记。对来自富集微卫星文库的 798 个甘蔗基因组 DNA 克隆进行测序，产生了 457 个含有微卫星重复基序的插入片段。超过 84% 的微卫星含有平均分别为 15 和 13 个重复基序的二核苷酸或三核苷酸重复。为 100 多个微卫星序列设计和合成了引物组，并在一组五个甘蔗品种上进行了测试。观察到等位基因数量所见证的杂合性和特定等位基因的 PCR 产物大小差异所见证的长度多态性。微卫星标记可能在甘蔗遗传和育种中有许多应用，包括种质分析、品种鉴定、亲本评估和标记辅助育种。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
Cultivated sugarcane varieties (Saccharum spp) are derived from complex interspecific hybridisations between the species S. spontaneum (2n = 40-128) and S. officinarum (2n = 60 or 80). To analyse this complex genome, the potential of microsatellite repeats as genetic markers in sugarcane with respect to their abundance, variability and ability to detect polymorphisms was investigated. A set of microsatellite markers for genome analysis in cultivated sugarcane was identified from an enriched genomic DNA library constructed from Saccharum sp, cv Q124. Sequencing of 798 sugarcane genomic DNA clones from an enriched microsatellite library, yielded 457 inserts containing microsatellite repeat motifs. Just over 84% of the microsatellites contained dinucleotide or trinucleotide repeats averaging 15 and 13 repeat motifs, respectively. Primer sets were designed and synthesised for over 100 microsatellite sequences and tested on a set of five sugarcane cultivars. Both, heterozygosity as witnessed by the number of alleles, and length polymorphisms as seen in the differences in PCR product size for a particular allele were observed. Microsatellite markers are likely to have many applications in sugarcane genetics and breeding including germplasm analysis, cultivar identification, parent evaluation and marker assisted breeding. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.