Characterization of flagella produced by clinical strains of Stenotrophomonas maltophilia
Characterization of flagella produced by clinical strains of Stenotrophomonas maltophilia
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DOI：
10.3201/eid0809.010535
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发表时间：
2002-09-01
影响因子：
11.8
通讯作者：
Girón, JA
中科院分区：
文献类型：
--
作者：
de Oliveira-Garcia, D;Dall'Agnol, M;Girón, JA
嗜麦芽窄食单胞菌是一种新出现的医院内病原体，与囊性纤维化、癌症和HIV患者的机会性感染相关。该微生物粘附于非生物表面，如医疗植入物和导管，对住院患者构成重大风险。参与这些细菌粘附的粘附表面因子在很大程度上是未知的，并且它们的鞭毛尚未被生化和抗原性地表征。对S.嗜麦芽窄食单胞菌临床菌株。鞭毛丝由一个38 kDa的亚基组成，SMFliC，其N-末端氨基酸序列的分析表明，相当大的序列同一性的鞭毛的粘质沙雷氏菌（78.6%），大肠杆菌，奇异变形杆菌，宋内志贺菌（71.4%），铜绿假单胞菌（57.2%）。通过扫描电子显微镜对粘附在塑料上的细菌进行的超微结构分析显示，细菌簇内具有鞭毛状结构，这表明当细菌在非生物表面上传播时产生鞭毛。
Stenotrophomonas maltophilia is an emerging nosocomial pathogen associated with opportunistic infections in patients with cystic fibrosis, cancer, and HIV. Adherence of this organism to abiotic surfaces such as medical implants and catheters represents a major risk for hospitalized patients. The adhesive surface factors involved in adherence of these bacteria are largely unknown, and their flagella have not yet been characterized biochemically and antigenically. We purified and characterized the flagella produced by S. maltophilia clinical strains. The flagella filaments are composed of a 38-kDa subunit, SMFliC, and analysis of its N-terminal amino acid sequence showed considerable sequence identity to the flagellins of Serratia marcescens (78.6%), Escherichia coli, Proteus mirabilis, Shigella sonnei (71.4%), and Pseudomonas aeruginosa (57.2%). Ultrastructural analysis by scanning electron microscopy of bacteria adhering to plastic showed flagellalike structures within the bacterial clusters, suggesting that flagella are produced as the bacteria spread on the abiotic surface.