RESCUE OF THE DROSOPHILA PHOTOTRANSDUCTION MUTATION TRP BY GERMLINE TRANSFORMATION
RESCUE OF THE DROSOPHILA PHOTOTRANSDUCTION MUTATION TRP BY GERMLINE TRANSFORMATION
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DOI：
10.1126/science.3933112
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
RUBIN, G
中科院分区：
文献类型：
--
作者：
MONTELL, C;JONES, K;RUBIN, G
光转导是视觉系统的光刺激感光细胞向神经系统发送电信号的过程。在光转导的初始事件之后的许多步骤，即视紫红质对光的吸收，是不明确的。果蝇（Drosophila melanogaster）提供了一种从遗传学角度剖析光传导的方法。突变如瞬时受体电位（trp）影响光转导的中间步骤。为了促进光转导的分子研究，分离了trp基因，并通过由P元件介导的7.1-色氨酸酶DNA片段的种系转化补充trp基因的突变trpCM等位基因来确认其身份。trp基因的表达在蛹发育后期开始，并且似乎仅限于眼睛和单眼。
Phototransduction is the process by which light-stimulated photoreceptor cells of the visual system send electrical signals to the nervous system. Many of the steps that follow the initial event in phototransduction, absorption of light by rhodopsin, are ill-defined. The fruitfly, Drosophila melanogaster, provides a means to dissect phototransduction genetically. Mutations such as transient receptor potential (trp) affect intermediate steps in phototransduction. In order to facilitate molecular studies of phototransduction, the trp gene was isolated and its identity was confirmed by complementing the mutant trpCM allele of the trp gene by P-element mediated germline transformation of a 7.1-kilobase DNA fragment. Expression of the trp gene begins late in pupal development and appears to be limited to the eyes and ocelli.