Insulin modulation of newly synthesized apolipoproteins B-100 and B-48 in human fetal intestine: Gene expression and mRNA editing are not involved
Insulin modulation of newly synthesized apolipoproteins B-100 and B-48 in human fetal intestine: Gene expression and mRNA editing are not involved
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DOI：
10.1016/0014-5793(96)00896-4
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发表时间：
1996-09-16
期刊：
影响因子：
3.5
通讯作者：
Menard, D
中科院分区：
文献类型：
--
作者：
Levy, E;Sinnett, D;Menard, D
We investigated insulin's effect on intestinal lipid transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion, Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion, When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin, HDL lipid content, apo A-1 synthesis and RNA level mere unaffected by insulin, Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.
