Biocompatibility of poly(etherurethane urea) containing dehydroepiandrosterone.
Biocompatibility of poly(etherurethane urea) containing dehydroepiandrosterone.
复制标题
含有脱氢表雄酮的聚醚氨酯脲的生物相容性。
DOI：
10.1002/(sici)1097-4636(199808)41:2
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Anderson,J
中科院分区：
文献类型：
--
作者：
Collier,T;Tan,J;Shive,M;Hasan,S;Hiltner,A;Anderson,J
聚醚脲（PEUU）弹性体具有广泛的机械性能和高的生物相容性，在临床上用于医疗应用。然而，PEUU的以太软段在长期使用中存在降解的可能性。为了延缓降解，添加了清除活性氧中间体的抗氧化剂。在这项研究中，我们加入了脱氢表雄酮（DHEA），它通过调节或下调粘附巨噬细胞活性的交替机制来延缓PEUUs的生物降解。研究了按重量计含有1%脱氢表雄酮、5%脱氢表雄酮和5%维生素E （α‐生育酚）的PEUU样品在体内和体外的生物相容性。生物相容性最初是通过检查炎症细胞渗出液来评估的。与不添加添加剂的PEUU和添加5%维生素E的PEUU相比，添加5%脱氢表雄酮的PEUU在第4天使白细胞渗出液总浓度降低。与其他材料相比，添加5% DHEA在7天时也使巨噬细胞粘附和FBGC形成降低。尽管存在这些短期影响，但所有材料在后期时间点（14,21和70天）的生物相容性相似。透射红外分析表明，植入3天后，70%以上的脱氢表雄酮已从样品中浸出。此外，通过衰减全反射傅里叶变换分析和扫描电镜，确定DHEA与维生素E不同，不提高PEUU的长期生物稳定性。脱氢表雄酮对炎症细胞活性的影响似乎是剂量依赖性的，对高负荷水平的脱氢表雄酮具有更好的体内生物相容性，但由于水溶性脱氢表雄酮从PEUU迅速扩散，总体效果有限。©1998 John Wiley & Sons, Inc中国生物医学工程学报，2016,33(2):481 - 481。
Poly(etherurethane urea) (PEUU) elastomers, with their broad range of mechanical properties and high biocompatibility, are used clinically for medical applications. However, the possibility exists for the ether soft segment of PEUU to degrade in long‐term uses. To retard degradation, antioxidants that scavenge reactive oxygen intermediates are added. In this study, we incorporated dehydroepiandrosterone (DHEA), which functions by the alternate mechanism of modulating or down‐regulating adherent macrophage activity, to retard the biodegradation of PEUUs. Biocompatibility of PEUU samples containing 1% DHEA, 5% DHEA, and 5% vitamin E (α‐tocopherol) by weight were studiedin vivoandin vitro. The biocompatibility was initially evaluated by examination of the inflammatory cellular exudate. Compared to PEUU without additives and PEUU with 5% vitamin E, the addition of 5% DHEA to PEUU caused a decrease in the total leukocyte exudate concentration at 4 days. The addition of 5% DHEA also caused lower macrophage adhesion and FBGC formation compared to the other materials at 7 days. Despite these short‐term effects, the biocompatibility at later time points (14, 21, and 70 days) was similar for all materials. Transmission infrared analysis of the materials revealed that more than 70% of the DHEA had leached out of the samples by 3 days implantation. Furthermore, through attenuated total reflectance Fourier transform analysis and scanning electron microscopy, it was determined that unlike vitamin E, DHEA did not enhance long‐term PEUU biostability. The effect of DHEA on inflammatory cell activity appeared to be dose dependent, with improved biocompatibilityin vivofor higher loading levels of DHEA, but the overall effect was limited owing to the rapid diffusion of the water‐soluble DHEA from the PEUU. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 192–201, 1998.