Neurochemical changes in the hippocampus of the Brown Norway rat during aging
Neurochemical changes in the hippocampus of the Brown Norway rat during aging
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DOI：
10.1016/s0197-4580(97)80314-4
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发表时间：
1997-05-01
影响因子：
4.2
通讯作者：
Fuxe, K
中科院分区：
文献类型：
--
作者：
Bhatnagar, M;Cintra, A;Fuxe, K
应用显微密度计和体视学技术研究了3、6、12、18、24、30和36月龄雄性Brown Norway大鼠海马的增龄效应。体视学分析的碱性成纤维细胞生长因子（bFGF）的免疫反应性胶质细胞在CA 1区的细胞数量呈年龄依赖性减少，从18个月的年龄。从24个月大开始，CA 3区、齿状回和CAI区的bFGF免疫反应性的具体平均灰度值降低。CA 1-CA 2区胶质细胞酸性蛋白和糖皮质激素受体（GR）免疫反应阳性细胞数在年龄组间无差异。然而，GR免疫反应性的强度在18个月大和更大的大鼠中降低。在任何年龄组的CA 1-CA 2区的盐皮质激素受体的免疫反应性没有变化。自发交替测试和反应在一个开放的领域没有发现显着差异的年龄组之间。这些发现提供了证据，有一个损失的神经GR免疫反应，但没有损失的GR免疫反应神经元，在CA 1-CA 2区的老年布朗挪威大鼠。衰老的特征还在于海马体中胶质源性生长因子（如bFGF）的大量缺乏。免疫反应性的变化与依赖于正常海马功能的选定行为的改变无关。(C)1997年爱思唯尔科学公司
Microdensitometrical and stereological techniques were applied to study the effects of aging on the hippocampus of 3-, 6-, 12-, 18-, 24-, 30-, and 36-month-old male Brown Norway rats. Stereological analysis of basic fibroblast growth factor (bFGF) immunoreactive glial cells in the CA1 area showed an age-dependent decrease in the number of cells, starting at 18 months of age. Specific mean gray values of the immunoreactivity for bFGF were reduced in the CA3 area, in the dentate gyrus, and in fields of the CAI area, starting at 24 months of age. There were no differences between the age groups in the number of glial fibrillary acidic protein or glucocorticoid receptor (GR) immunoreactive cells of the CA1-CA2 areas. However, the intensity of the GR immunoreactivity was decreased in the 18-month-old and older rats. No changes in the immunoreactivity for the mineralocorticoid receptor were observed in the CA1-CA2 areas of any of the age groups. Spontaneous alternation test and reactivity in an open field did not reveal marked differences between the age groups. These findings give evidence that there is a loss of neural GR immunoreactivity, but no loss of GR immunoreactive neurons, in the CA1-CA2 areas of the aged Brown Norway rat. Aging may also be characterized by substantial deficits of glially derived growth factors, such as bFGF in the hippocampus. The changes in immunoreactivities were not correlated to alterations in selected behaviors dependent on normal hippocampal function. (C) 1997 Elsevier Science Inc.