Jinggangmycin increases fecundity of the brown planthopper, Nilaparvata lugens (Stal) via fatty acid synthase gene expression
Jinggangmycin increases fecundity of the brown planthopper, Nilaparvata lugens (Stal) via fatty acid synthase gene expression
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DOI：
10.1016/j.jprot.2015.09.022
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发表时间：
2016-01-01
影响因子：
3.3
通讯作者：
Wu, Jincai
中科院分区：
文献类型：
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作者：
Li, Lei;Jiang, Yiping;Wu, Jincai
井冈霉素(JGM)是中国防治水稻纹枯病的主要药剂。JGM还促进褐飞虱(BPH)的繁殖。然而，到目前为止，这种增强的分子机制尚不清楚。我们的相关报告记录了JGM叶面喷雾对卵巢蛋白质含量的影响。在这里，我们使用等压相对和绝对定量标记(ITRAQ)方法来分析喷雾剂(JGM-S)和外用(JGM-T)后BPH女性的卵巢蛋白。与S对照组(S对照组)相比，JGM-T组有267个蛋白质表达差异(向上箭头130个，向下箭头137个)，其中22个蛋白质在两组中均上调。比较JGM-S组和JGM-T组，差异表达的蛋白质有114个(向上箭头62个，向下箭头52个)。基于脂肪酸的生物学意义、途径注释和富集性分析，我们设计了一个dsRNA结构来沉默一个编码脂肪酸合成酶(FAS)的基因。JGM-S组较S对照组、JGM-S组与JGM-T组Fas表达较高。与对照组相比，dsFAS处理组雌鼠的繁殖力降低了约46%，卵巢和脂肪体脂肪酸浓度降低。我们推断，Fas提供急需的脂肪酸，以支持JGM增强的BPH的繁殖力。(C)由爱思唯尔出版的2015年。
The antibiotic jinggangmycin (JGM) is mainly used in controlling the rice sheath blight, Rhizoctonia solani, in China. JGM also enhances reproduction of the brown planthopper (BPH), Nilaparvata lugens (Stal). To date, however, molecular mechanisms of the enhancement are unclear. Our related report documented the influence of foliar JGM sprays on ovarian protein content. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) protocols to analyze ovarian proteins of BPH females following JGM spray (JGM-S) and topical application (JGM-T). We recorded changes in expression of 284 proteins (142 up arrow and 142 down arrow) in JGM-S compared to the JGM-S control group (S-control); 267 proteins were differentially expressed (130 up arrow and 137 down arrow) in JGM-T compared to the JGM-T control group (T-control), of which, 22 proteins were up-regulated in both groups. Comparing the JGM-S to the JGM-T group, 114 proteins were differentially expressed (62 up arrow and 52 down arrow). Based on the biological significance of fatty acids, pathway annotation and enrichment analysis, we designed a dsRNA construct to silence a gene encoding fatty acid synthase (FAS). FAS was more highly expressed in JGM-S vs S-control and JGM-S vs JGM-T groups. The dsFAS treatment reduced fecundity by about 46% and reduced ovarian and fat body fatty acid concentrations in JGM-S-treated females relative to controls. We infer FAS provides critically needed fatty acids to support JGM-enhanced fecundity in BPH. (C) 2015 Published by Elsevier B.V.