Direct transmission of H-pylori from challenged to nonchallenged mice in a single cage
Direct transmission of H-pylori from challenged to nonchallenged mice in a single cage
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DOI：
10.1007/s10620-005-2710-x
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发表时间：
2005-06-01
影响因子：
3.1
通讯作者：
Sugiyama, T
中科院分区：
文献类型：
--
作者：
Karita, M;Matsumoto, S;Sugiyama, T
为了了解幽门螺杆菌是否会从受感染的小鼠直接传播到未受感染的小鼠，我们使用我们之前开发的小鼠模型对系统进行了测试。六只裸鼠接受幽门螺杆菌接种攻击；一组由接种后1周的一只受攻击的裸鼠组成，与四只未受攻击的裸鼠在一个笼子中饲养。对于单个笼子，使用聚碳酸酯笼子或网状地板笼子。然后三组被关在聚碳酸酯笼子里，另外三组被关在网状地板笼子里，以避免幽门螺杆菌通过粪便传播。共饲养1、2或3周后，处死所有小鼠，通过培养或PCR确定胃、唾液和粪便中幽门螺杆菌的存在以及幽门螺杆菌相关性胃炎。使用来自受攻击和未受攻击小鼠的分离菌株的不同引物对 RAPD 指纹图谱进行比较，以了解传播菌株的起源。在幽门螺杆菌攻击和未攻击小鼠共饲养3周后，在聚碳酸酯笼中的12只未攻击小鼠中的3只和在钢网地板笼中的12只未攻击小鼠中的2只中检测到幽门螺杆菌。在聚碳酸酯笼中的两只未受到攻击的感染小鼠的唾液或粪便中检测到幽门螺杆菌。此外，使用从两个笼子中的五只未攻击的感染小鼠分离的总共五种菌株的不同引物进行的RAPD指纹分析显示出与攻击菌株和从攻击小鼠分离的菌株相同的模式和一致性。研究表明，亲密互动是幽门螺杆菌通过唾液和粪便传播的原因。
To understand whether direct transmission of H. pylori occurs from infected mouse to noninfected mouse, the system using a mouse model we developed previously was tested. Six nude mice were challenged with H. pylori inocula; one group consisted of one challenged nude mouse 1 week after inoculation raised with four nonchallenged nude mice in a single cage. For the single cage, a polycarbonate cage or a mesh-floor cage was used. Then three groups were kept in a polycarbonate cage and the other three groups kept in a mesh-floor cage to avoid H. pylori transmission through stool. After coraising for 1, 2, or 3 weeks, all mice were sacrificed to determine the existence of H. pylori in the stomach, saliva, and stool by culture or PCR and H. pylori-associated gastritis. RAPD fingerprinting patterns using different primers of isolated strains from challenged and nonchallenged mice were compared to understand the origin of transmitted strains. During 3 weeks after coraising of H. pylori challenged and nonchallenged mice, H. pylori was detected in the stomachs in 3 of 12 nonchallenged mice in the polycarbonate cage and in 2 of 12 nonchallenged mice in the cage with a steel mesh floor. H. pylori was detected from saliva or stool in two nonchallenged, infected mice in the polycarbonate cage. Moreover, RAPD fingerprinting using different primers of the total five strains isolated from five nonchallenged, infected mice in both cages showed the same pattern and concordance with that of the challenged strain and the strains isolated from challenged mice. It is demonstrated that intimate interaction is the cause of H. pylori transmission via saliva and stool.