Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases
Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases
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DOI：
10.1073/pnas.181141598
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发表时间：
2001-09-11
影响因子：
11.1
通讯作者：
Bleecker, AB
中科院分区：
文献类型：
--
作者：
Shiu, SH;Bleecker, AB
植物受体样激酶（RLKs）是具有预测信号序列、单一跨膜区和胞质激酶结构域的蛋白质。受体样激酶是一个基因大家族，约有610个成员，占拟南芥蛋白质编码基因的2.5%。我们已经将该家族的成员根据胞外结构域的身份和亚家族成员的激酶结构域之间的系统发育关系分类为亚家族。令人惊讶的是，这个结构上确定的基因组是单系相对于激酶结构域相比，与其他真核生物激酶家族。在扩展分析中，动物受体激酶、Raf激酶、植物RLK和动物受体酪氨酸激酶形成了一个得到充分支持的组，在丝氨酸/苏氨酸/酪氨酸激酶超家族内共享共同的起源。在动物激酶序列中，果蝇Pelle和相关的细胞质激酶属于植物RLK进化枝，我们现在将其定义为RLK/Pelle家族。陆地植物表达序列标签记录的调查显示，苔藓，蕨类植物，针叶树，开花植物有类似的百分比表示RLK/Pelle同源序列标签，这表明该基因家族的大小可能已经接近今天的水平之前，多样化的陆地植物谱系。四个RLK亚家族在拟南芥染色体上的分布模式表明，该基因家族的扩展部分是拟南芥基因组重复和重排以及产生串联重复序列的结果。
Plant receptor-like kinases (RLKs) are proteins with a predicted signal sequence, single transmembrane region, and cytoplasmic kinase domain. Receptor-like kinases belong to a large gene family with at least 610 members that represent nearly 2.5% of Arabidopsis protein coding genes. We have categorized members of this family into subfamilies based on both the identity of the extracellular domains and the phylogenetic relationships between the kinase domains of subfamily members. Surprisingly, this structurally defined group of genes is monophyletic with respect to kinase domains when compared with the other eukaryotic kinase families. In an extended analysis, animal receptor kinases, Raf kinases, plant RLKs, and animal receptor tyrosine kinases form a well supported group sharing a common origin within the superfamily of serine/threonine/tyrosine kinases. Among animal kinase sequences, Drosophila Pelle and related cytoplasmic kinases fall within the plant RLK clade, which we now define as the RLK/Pelle family. A survey of expressed sequence tag records for land plants reveals that mosses, ferns, conifers, and flowering plants have similar percentages of expressed sequence tags representing RLK/Pelle homologs, suggesting that the size of this gene family may have been close to the present-day level before the diversification of land plant lineages. The distribution pattern of four RLK subfamilies on Arabidopsis chromosomes indicates that the expansion of this gene family is partly a consequence of duplication and reshuffling of the Arabidopsis genome and of the generation of tandem repeats.