Effects of cranberry components on IL-1β-stimulated production of IL-6, IL-8 and VEGF by human TMJ synovial fibroblasts
Effects of cranberry components on IL-1β-stimulated production of IL-6, IL-8 and VEGF by human TMJ synovial fibroblasts
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DOI：
10.1016/j.archoralbio.2016.04.005
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发表时间：
2016-08-01
影响因子：
3
通讯作者：
Blumer, Adam
中科院分区：
文献类型：
--
作者：
Tipton, David A.;Christian, James;Blumer, Adam
目的：颞下颌关节骨关节炎（OA）是一种以关节软骨退化和继发性炎症改变为特征的疾病。白细胞介素-1 β（IL-1 β）刺激IL-6，IL-8和血管内皮生长因子（VEGF）在TMJ的滑液与内部紊乱和骨的变化。蔓越莓（Vaccinium macrocarpon）含有多酚化合物，可抑制牙龈细胞对几种刺激物产生促炎分子。本研究探讨了蔓越莓成分对IL-1 β刺激的IL-6，IL-8和VEGF的生产由人TMJ滑膜成纤维细胞样cells.Design：蔓越莓高分子量非透析材料（NDM）是来自蔓越莓汁。将来自患有退行性OA的关节和未退化的强直TMJ的人TMJ滑膜成纤维细胞样细胞与IL-1 β（0.001-1 nM）+/- NDM（25-250 μ g/ml）一起孵育（预孵育2小时）。通过线粒体酶的活性评估活力。通过ELISA测量培养上清液中的IL-6、IL-8和VEGF;通过与特异性寡核苷酸结合测量核提取物中的NF-κ B和AP-1转录因子。数据分析：结果：NDM不影响细胞活力，但在所有细胞系中抑制IL-1 β刺激的IL-6、IL-8和VEGF的产生（p
Objective: Osteoarthritis (OA) in the TMJ is characterized by deterioration of articular cartilage and secondary inflammatory changes. Interleukin-1 beta (IL-1 beta) stimulates IL-6, IL-8, and vascular endothelial growth factor (VEGF) in synovial fluid of TMJ with internal derangement and bony changes. The cranberry (Vaccinium macrocarpon) contains polyphenolic compounds that inhibit production of pro-inflammatory molecules by gingival cells in response to several stimulators. This study examined effects of cranberry components on IL-1 beta-stimulated IL-6, IL-8, and VEGF production by human TMJ synovial fibroblast-like cells.Design: Cranberry high molecular weight non-dialyzable material (NDM) was derived from cranberry juice. Human TMJ synovial fibroblast-like cells from joints with degenerative OA and an ankylosed TMJ without degeneration were incubated with IL-1 beta (0.001-1 nM) +/- NDM (25-250 mu g/ml) (2 h preincubation). Viability was assessed via activity of a mitochondria] enzyme. IL-6, IL-8, and VEGF in culture supernatants were measured by ELISA; NF-kappa B and AP-1 transcription factors were measured in nuclear extracts via binding to specific oligonucleotides. Data analysis: ANOVA and Scheffe's F procedure for post hoc comparisons.Results: NDM did not affect cell viability but inhibited IL-1 beta stimulated IL-6, IL-8, and VEGF production in all cell lines (p