Construction and characterization of recombinant vaccinia viruses co-expressing a respiratory syncytial virus protein and a cytokine.
Construction and characterization of recombinant vaccinia viruses co-expressing a respiratory syncytial virus protein and a cytokine.
复制标题
共表达呼吸道合胞病毒蛋白和细胞因子的重组痘苗病毒的构建和表征。
DOI：
10.1099/0022-1317-82-9-2107
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Graham,BarneyS
中科院分区：
文献类型：
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作者：
Johnson,TeresaR;Fischer,JulieE;Graham,BarneyS
重组牛痘病毒是充分表征的工具，可用于定义疫苗配制和递送的新方法。虽然免疫介质的载体共表达对于优化疫苗诱导的免疫应答的组成具有巨大的潜力，但也必须考虑对抗原表达和载体抗原性的影响。IL-4的共表达增加了牛痘病毒载体滴度，而IFN-γ的共表达减少了感染某些重组病毒的BALB/c小鼠和C57 BL/6小鼠中牛痘病毒的复制。在用表达RSV G糖蛋白和IFN-γ的牛痘病毒免疫的小鼠中，对呼吸道合胞病毒（RSV）攻击的保护作用相似，尽管载体的复制效率降低。这些数据证明了载体表达的细胞因子影响载体毒力和指导所选免疫应答发展的能力。这表明细胞因子和其他免疫调节剂的共表达具有提高疫苗载体安全性的潜力，同时提高疫苗抗原的免疫原性。
Recombinant vaccinia viruses are well-characterized tools that can be used to define novel approaches to vaccine formulation and delivery. While vector co-expression of immune mediators has enormous potential for optimizing the composition of vaccine-induced immune responses, the impact on antigen expression and vector antigenicity must also be considered. Co-expression of IL-4 increased vaccinia virus vector titres, while IFN-γ co-expression reduced vaccinia virus replication in BALB/c mice and in C57BL/6 mice infected with some recombinant viruses. Protection against respiratory syncytial virus (RSV) challenge was similar in mice immunized with vaccinia virus expressing RSV G glycoprotein and IFN-γ, even though the replication efficiency of the vector was diminished. These data demonstrate the ability of vector-expressed cytokine to influence the virulence of the vector and to direct the development of selected immune responses. This suggests that the co-expression of cytokines and other immunomodulators has the potential to improve the safety of vaccine vectors while improving the immunogenicity of vaccine antigens.