TUBULIN BIOSYNTHESIS IN THE DEVELOPMENTAL CYCLE OF A PARASITIC PROTOZOAN, LEISHMANIA-MEXICANA - CHANGES DURING DIFFERENTIATION OF MOTILE AND NON-MOTILE STAGES
TUBULIN BIOSYNTHESIS IN THE DEVELOPMENTAL CYCLE OF A PARASITIC PROTOZOAN, LEISHMANIA-MEXICANA - CHANGES DURING DIFFERENTIATION OF MOTILE AND NON-MOTILE STAGES
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DOI：
10.1073/pnas.78.12.7624
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
CHANG, KP
中科院分区：
文献类型：
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作者：
FONG, D;CHANG, KP
在体外研究了寄生原生动物墨西哥河鼠（L. mexicana amazonensis）传播周期中的细胞分化。在35度下，有鞭毛的活动无纺体在7天内转变为无活动无纺体。小鼠巨噬细胞系J774G8细胞内C的表达。在培养基199加胎牛血清中，27度时细胞外发生反向转化。C在2天内。用[35S]蛋氨酸标记的利什曼原虫在转化过程中，平板凝胶电泳显示蛋白质条带模式的变化。具有表观分子量的2种蛋白的强度。55,000在无纺布向promastigote分化过程中增加，在反向转化过程中减少。这两种蛋白与α蛋白大致同源。-和。beta。鞭毛衣单胞菌-微管蛋白的二维凝胶电泳。较低的波段进一步确定为。beta。用兔抗血清免疫沉淀-微管蛋白。轴状衣单胞菌的微管蛋白。微管蛋白的生物合成变化与转化过程中鞭毛微管和细胞骨架的形态变化有关。
Cytodifferentiation in the transmission cycle of the parasitic protozoan L. mexicana amazonensis was studied in vitro. The flagellated motile promastigotes transform into the nonmotile amastigotes in 7 days at 35.degree. C intracellularly in the murine macrophage line J774G8. In medium 199 plus fetal bovine serum, the reverse transformation occurs extracellularly at 27.degree. C in 2 days. Slab gel electrophoresis of Leishmania labeled with [35S]methionine during transformation revealed changes in protein banding patterns. The intensity of 2 protein species with apparent MW .apprxeq. 55,000 increased in the amastigote-to-promastigote differentiation and decreased during the reverse transformation. These 2 protein species comigrated approximately with .alpha.- and .beta.-tubulin of Chlamydomonas flagella in 2-dimensional gel electrophoresis. The lower band was further identified as .beta.-tubulin by immunoprecipitation using rabbit antiserum specific to the .beta.-tubulin of Chlamydomonas axonemes. The biosynthetic change of tubulin correlated with the morphological change of microtubules in leishmanial flagella and cytoskeleton during transformation.