Improvement of chemical analysis of antibiotics. XII. Simultaneous analysis of seven tetracyclines in honey.
Improvement of chemical analysis of antibiotics. XII. Simultaneous analysis of seven tetracyclines in honey.
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抗生素化学分析的改进。
DOI：
10.1016/s0021-9673(01)81619-8
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Makoto Suzuki
中科院分区：
文献类型：
--
作者：
H. Oka;Y. Ikai;N. Kawamura;Keiichi Uno;M. Yamada;Ken;Makoto Suzuki
建立了一种同时测定蜂蜜中土霉素、四环素、金霉素、强力霉素、美他环素、去甲金霉素和米诺环素残留量的方法。该方法采用薄层层析法，不需要特殊仪器，即可检测蜂蜜中0.1ppm的四环素类抗生素，采用高效液相色谱法定量，回收率为83.7-99.6%，变异系数为0.9-4.3%。
An analytical system for the simultaneous determination of residual oxytetracycline, tetracycline, chlortetracycline, doxycycline, methacycline, demethylchlortetracycline and minocycline in honey has been established by a combination of simple thin-layer chromatographic (TLC) and precise high-performance liquid chromatographic (HPLC) methods. In this system, screening by TLC can detect tetracyclines (TCs) at a level of 0.1 ppm in honey without the need for special equipment, and the quantitative method by HPLC can determine TCs with good recovery (83.7–99.6%) and coefficient variation (0.9–4.3%).