Reactivity of Trametes laccases with fatty and resin acids
Reactivity of Trametes laccases with fatty and resin acids
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栓菌漆酶与脂肪酸和树脂酸的反应性
DOI：
10.1007/s002530000532
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发表时间：
2001
影响因子：
5
通讯作者：
J. Buchert
中科院分区：
文献类型：
--
作者：
Stina Karlsson;B. Holmbom;P. Spetz;A. Mustranta;J. Buchert
抽象的。通常称为木沥青或木树脂的亲脂性提取物会对纸机的运行性能和产品质量产生负面影响。亲脂性浸提物主要由脂肪酸、树脂酸、甾醇、甾醇酯和甘油三酯组成。在这项工作中，漆酶的脂肪酸和树脂酸的改性的适用性进行了研究，使用两个模型馏分。在处理中，树脂和脂肪酸分散体用两种不同的漆酶，即来自毛栓菌和T.绒毛。不同的色谱方法被用来阐明漆酶处理对脂肪酸和树脂酸的化学性质的影响。这两种漆酶都能在一定程度上修饰脂肪酸和树脂酸。在脂肪酸的情况下，观察到亚油酸、油酸和皮诺敛酸的量减少，而树脂酸的改性导致共轭树脂酸的量减少。
Abstract. Lipophilic extractives commonly referred to as wood pitch or wood resin can have a negative impact on paper machine runnability and product quality. The lipophilic extractives are composed mainly of fatty acids, resin acids, sterols, steryl esters and triglycerides. In this work, the suitability of laccases for the modification of fatty and resin acids was studied, using two model fractions. In the treatments, resin and fatty acid dispersions were treated with two different laccases, i.e. laccases from Trametes hirsuta and T. villosa. Different chromatographic methods were used to elucidate the effects of laccase treatments on the chemistry of the fatty and resin acids. Both laccases were able to modify the fatty and resin acids to some extent. In the case of fatty acids, a decrease in the amount of linoleic, oleic and pinolenic acids was observed, whereas the modification of resin acids resulted in a reduced amount of conjugated resin acids.