Functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat
Functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat
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DOI：
10.1210/en.141.10.3832
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发表时间：
2000-10-01
期刊：
影响因子：
4.8
通讯作者：
Buijs, RM
中科院分区：
文献类型：
--
作者：
Kalsbeek, A;Fliers, E;Buijs, RM
通过心房插管频繁采血，揭示了雄性和雌性 Wistar 大鼠 TSH 和甲状腺激素的每日节律。生物钟（即视交叉上核 (SCN)）的热消融消除了循环 TSH 和甲状腺激素的昼夜峰值。此外，SCN 损伤导致 24 小时平均 T-4 浓度明显下降。与 TSH 相比，SCN 损伤对甲状腺激素的影响更为明显，这表明除神经内分泌下丘脑垂体-甲状腺轴外还有 SCN 控制途径。逆行跨神经元病毒追踪用于识别中枢神经系统中控制甲状腺自主神经支配的神经元的类型和定位。在脊髓和脑干中，交感神经和副交感运动神经元都被标记。通过改变接种后的存活时间，可以跟踪病毒感染的进展。随后，伪狂犬病病毒（PRV）感染了多个脑干细胞群中的神经元、下丘脑室旁核（PVN）和杏仁核中央核（二级标记）。 PVN 中感染 PRV 的神经元中含有 TRH 细胞。在几个下丘脑细胞群中发现了三级神经元，其中包括视交叉上核（SCN）。因此，我们建议； SCN 对甲状腺活动具有双重控制机制，一方面影响 TSH 释放的神经内分泌控制，另一方面影响甲状腺的自主输入。
Frequent blood sampling via intraatrial cannula revealed daily rhythms of TSH and thyroid hormones in both male and female Wistar rats. Thermic ablation of the biological clock, i.e. the suprachiasmatic nucleus (SCN), eliminated the diurnal peak in circulating TSH and thyroid hormones. In addition, SCN lesions produced a clear decrease of 24-h mean T-4 concentrations. A more pronounced effect of SCN-lesions on thyroid hormones, as opposed to TSH, suggested routes of SCN control additional to the neuroendocrine hypothalamopituitary-thyroid axis. Retrograde, transneuronal virus tracing was used to identify the type and localization of neurons in the central nervous system that control the autonomic innervation of the thyroid gland. In the spinal cord and brain stem, both the sympathetic and parasympathetic motorneurons were labeled, By varying the postinoculation survival time, it was possible to follow the viral infection as it proceeded. Subsequently, the pseudorabies virus (PRV) infected neurons in several brain stem cell groups, the paraventricular nucleus of the hypothalamus (PVN) and the central nucleus of the amygdala (second order labeling). Among PRV-infected neurons in the PVN were TRH-containing cells. Third order neurons were found in several hypothalamic cell groups, among which was the SCN. Therefore, we propose that; the SCN has a dual control mechanism for thyroid activity by affecting neuroendocrine control of TSH release on the one hand and the autonomic input to the thyroid gland on the other.