HOMOLOGS OF THE MOUSE BRACHYURY GENE ARE INVOLVED IN THE SPECIFICATION OF POSTERIOR TERMINAL STRUCTURES IN DROSOPHILA, TRIBOLIUM, AND LOCUSTA
HOMOLOGS OF THE MOUSE BRACHYURY GENE ARE INVOLVED IN THE SPECIFICATION OF POSTERIOR TERMINAL STRUCTURES IN DROSOPHILA, TRIBOLIUM, AND LOCUSTA
复制标题
DOI：
10.1101/gad.8.18.2137
复制
发表时间：
1994-09-15
影响因子：
10.5
通讯作者：
REUTER, R
中科院分区：
文献类型：
--
作者：
KISPERT, A;HERRMANN, BG;REUTER, R
Brachyury（T）基因是脊椎动物脊索分化所必需的。我们已经鉴定了果蝇的一个基因，T相关基因（Trg），在T的DNA结合结构域（200个氨基酸）内与T具有高度相似性。Trg在整个胚胎发生过程中表达，首先在后肠原基中的胚盘阶段在末端缺口基因tll和hkb的控制下表达，然后在分化中的后肠中表达直至胚胎发生结束。缺乏Trg g g的果蝇胚胎不形成后肠，这是一种可以通过Trg转基因拯救的表型。因此，T和Trg的共同特征是它们需要指定单个胚胎结构的发育。Trg的同源物也在拟谷盗和蝗虫胚胎的发育中的后肠中表达，表明Trg在昆虫中的高度保守的功能。这种保守性和T和Trg的高度相似性提出了昆虫后肠和脊索动物脊索共同进化起源的问题。
The Brachyury (T) gene is required for notochord differentiation in vertebrates. We have identified a Drosophila gene, the T-related gene (Trg), with high similarity to T within a stretch of similar to 200 amino acids, the DNA-binding domain of T. Trg is expressed throughout embryogenesis, first at the blastoderm stage in the hindgut primordium under the control of the terminal gap genes tll and hkb, and then until the end of embryogenesis in the differentiating hindgut. Drosophila embryos deficient for Trg g do not form the hindgut, a phenotype that can be rescued by a Trg transgene. Thus, a common feature of T and Trg is their requirement in specifying the development of a single embryonic structure. Homologs of Trg are also expressed in the developing hindgut of Tribolium and Locusta embryos suggesting a highly conserved function of Trg in insects. This conservation and the high similarity of T and Trg raise the question of a common evolutionary origin of tire hindgut of insects and the notochord of chordates.