Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles
Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles
复制标题
基于金纳米粒子催化组装和计数的超灵敏无扩增蛋白激酶检测
DOI：
10.1039/c9cc00131j
复制
发表时间：
2019
影响因子：
4.9
通讯作者：
Liu Baohong
中科院分区：
文献类型：
--
作者：
Tian Tongtong;Yao Yuanyuan;Yang Beibei;Zhang Kun;Liu Baohong
A single-particle enumeration method based on phosphorylation-directed in situ assembly of gold nanoparticles is developed for the ultrasensitive sensing of cellular protein kinase A activity. In comparison to existing strategies, the proposed new method demonstrates five orders of linear range and improves the detection limit up to 10-to-1000 fold without the involvement of target amplification.
