Direct Sequencing of In Vitro Amplified DNA
Direct Sequencing of In Vitro Amplified DNA
复制标题
体外扩增 DNA 的直接测序
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
U. Gyllensten
中科院分区：
文献类型：
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作者：
U. Gyllensten
该方法取代了大多数重复类型的分子克隆和用于测序的模板制备。与自动测序技术相结合，聚合酶链式反应将提供产生核苷酸序列信息的最快和最有效的手段。本章的目的是回顾测序模板的制备方法和对PCR产物进行直接测序的方法。
The PCR method has provided a substitute for most of the repetitive types of molecular cloning and template preparation for sequencing. In combination with automated sequencing techniques, PCR will provide the fastest and most efficient means of generating nucleotide acid sequence information. The purpose of this chapter is to review methods for preparation of sequencing templates and performing direct sequencing of PCR products.