Percentages of PD-1C+D4CT cells and PD-L1+DCs are increased and sPD-1 level is elevated in patients with immune thrombocytopenia
Percentages of PD-1C+D4CT cells and PD-L1+DCs are increased and sPD-1 level is elevated in patients with immune thrombocytopenia
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免疫性血小板减少症患者的 PD-1C D4CT 细胞和 PD-L1 DC 百分比增加，sPD-1 水平升高
DOI：
10.1080/21645515.2017.1342913
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发表时间：
2018
影响因子：
4.8
通讯作者：
Xinhong Guo
中科院分区：
文献类型：
--
作者：
Yingying Wang;Nannan Pang;Xinyou Wang;Ying Liu;Xiujuan Wang;Lei Wang;Mingling Sun;Halida Yasen;Fang Zhao;Wenxia Fan;Jianbing Ding;Xinhong Guo
检测免疫性血小板减少症(ITP)患者外周血中程序性死亡(PD)-1/程序性死亡配体-1(PD-L1)共刺激分子、可溶性形式SPD-1的表达，探讨其与细胞因子分泌的关系。本研究共纳入35例ITP患者。选择本院健康体检者20例为对照组。采集所有ITP患者和健康受试者的外周血。采用流式细胞仪检测ITP患者和健康体检者外周血中PD-1+CD4C+T细胞和PD-L1+DC的比例。采用酶联免疫吸附试验检测ITP患者和健康体检者外周血中干扰素-g、白介素17和sPD-1的浓度。ITP患者治疗前外周血中PD-1+CD4+T细胞和PD-L1+DC百分率明显高于正常对照组，但与治疗后无明显差异。ITP患者治疗前血清干扰素-γ、IL-17、Spd-1水平显著高于正常对照组，治疗后显著下降。SPD-1浓度与干扰素-g浓度呈正相关，与血小板计数呈负相关。ITP患者外周血中PD-1CCD4CT细胞和PD-L1CDCs的百分率均高于正常人，但血中Spd-1浓度升高阻断了PD-1/PD-L1信号通路，导致Th细胞功能未受影响。血中干扰素-γ和白介素17水平升高可能参与了ITP的发生发展。
The present study is to measure the expression of programmed death (PD)-1 / programmed death ligand-1(PD-L1) negative costimulatory molecules, soluble format sPD-1 in patients with immune thrombocytopenia (ITP), and to investigate their correlation with the secretion of cytokines. A total of 35 patients with ITP were included in the present study. Twenty healthy subjects who received physical examination at our hospital were included as control group. Peripheral blood was collected from all ITP patients and healthy subjects. Flow cytometry was performed to determine the percentages of PD-1+CD4C+T cells and PD-L1+DCs in ITP patients and healthy subjects. Enzyme-linked immunosorbent assay was performed to measure the concentrations of interferon (IFN)-g, interleukin (IL)-17 and sPD-1 in peripheral blood from ITP patients and healthy subjects. Percentages of PD-1+CD4+T cells and PD-L1+DCs in peripheral blood from ITP patients before treatment were significantly higher than that from healthy subjects, but were not different from those after treatment. Serum concentrations of IFN-g, IL-17 and sPD-1 in ITP patients before treatment were significantly higher than those in healthy subjects, and these concentrations were significantly reduced after treatment. The concentration of sPD-1 was positively correlated with the concentration of IFN-g, and negatively correlated with platelet count. Percentages of PD-1CCD4CT cells and PD-L1CDCs in ITP patients are higher than those in healthy subjects, but elevated sPD-1 concentration in the blood blocks PD-1/PD-L1 signaling pathway, leading to unaffected Th cel function. Elevated concentrations of IFN-g and IL-17 in the blood may participate in the occurrence and development of ITP.