Surface modification of silicone intraocular lens by 2-methacryloyloxyethyl phosphoryl-choline binding to reduce Staphylococcus epidermidis adherence
Surface modification of silicone intraocular lens by 2-methacryloyloxyethyl phosphoryl-choline binding to reduce Staphylococcus epidermidis adherence
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DOI：
10.1111/j.1442-9071.2007.01516.x
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发表时间：
2007-07-01
影响因子：
4
通讯作者：
Xu, Zhi-Kang
中科院分区：
文献类型：
--
作者：
Huang, Xiao-Dan;Yao, Ke;Xu, Zhi-Kang
目的：分析表皮葡萄球菌对2-甲基丙烯酰氧乙基磷酰胆碱（MPC）修饰的硅胶人工晶状体（IOL）的体外粘附情况。方法：采用空气等离子体处理对测试IOL进行修饰，使MPC与表面结合。对照 IOL 未进行修改。通过 X 射线光电子能谱 (XPS) 分析 IOL 表面的化学变化，以确认 MPC 的共价结合。 IOL的亲水性通过测量水接触角来确定。采用超声处理释放的活粘附细菌直接计数和扫描电子显微镜 (SEM) 两种不同的技术来观察和比较孵育 1 小时和 18 小时后表皮葡萄球菌对 IOL 的粘附情况。 结果：XPS 分析证实测试 IOL 已用 MPC 进行表面修饰。通过表面修饰提高了人工晶状体的亲水性，并且经过MPC修饰的人工晶状体在1小时的潜伏期后表现出表皮葡萄球菌的粘附显着降低（P = 0.002）。 SEM结果表明，MPC修饰还抑制了培养18小时后细菌的积累和生物膜的产生。结论：MPC修饰的亲水性硅胶人工晶状体减少了细菌的粘附和定植，从而可能有助于降低术后眼内炎的发生率。
Purpose: To analyse the in vitro adherence of Staphylococcus epidermidis to the 2-methacryloyl oxyethyl phosphorylcholine (MPC)-modified silicone intraocular lens (IOL).Methods: The test IOLs were modified by using an air plasma treatment to bind MPC to the surface. The control IOLs were not modified. Chemical changes on the IOL surface were analysed by X-ray photoelectron spectroscopy (XPS) to confirm the covalent binding of MPC. IOL hydrophilicity was determined by measuring the water contact angle. Two different techniques, direct counting of viable adherent bacteria released by sonication, and scanning electron microscopy (SEM), were used to observe and compare the adherence of S. epidermidis to the IOLs after 1- and 18-h incubation.Results: XPS analysis confirmed that the test IOLs were surface-modified with MPC. The hydrophilicity of the IOLs was improved by surface modification, and the MPC-modified IOLs exhibited significantly reduced adhesion of S. epidermidis (P = 0.002) after an incubation period of 1 h. The SEM results showed that the MPC modification also suppressed the accumulation of bacteria and biofilm production after 18 h incubation.COnclusions: MPC-modified hydrophilic silicone IOLs reduce bacterial adherence and colonization, and thus may help reduce the incidence of postoperative endophthalmitis.