A simplification of Heukeshoven and Dernick's silver staining of proteins
A simplification of Heukeshoven and Dernick's silver staining of proteins
复制标题
Heukeshoven 和 Dernick 蛋白质银染色的简化
DOI:
10.1002/elps.1150080308
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发表时间:
1987
期刊:
影响因子:
2.9
通讯作者:
D. de Vienne
中科院分区:
文献类型:
--
作者:
C. Damerval;M. Guilloux;J. Blaisonneau;D. de Vienne
The procedure of Heukeshoven and Dernick for silver staining of proteins in polyacrylamide gels is simplified by applying Farmer's redücer before impregnation with silver nitrate and rinsing the gel in 2.5 % sodium carbonate instead of distilled water. This modification yields pattern of good contrast without a destaining‐restaining step.