Alternative splicing of circadian clock genes correlates with temperature in field-grown sugarcane
Alternative splicing of circadian clock genes correlates with temperature in field-grown sugarcane
复制标题
生物钟基因的选择性剪接与田间种植甘蔗的温度相关
DOI：
10.1101/718890
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Dantas L
中科院分区：
文献类型：
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作者：
Dantas L
选择性剪接（AS）是一种从同一基因的前体mrna （pre-mRNAs）中产生不同成熟转录本的机制。在植物中，广泛的生理和代谢事件与AS有关，以及对温度变化的快速反应。拟南芥中约60%的内含子基因、水稻中46%的内含子基因和玉米中38%的内含子基因中存在AS，并且在生物钟基因中广泛存在。对于AS如何影响C4植物的生物钟知之甚少，比如商业甘蔗，一种具有复杂杂交基因组的C4作物。这项工作旨在确定核心生物钟基因中的AS事件，以及这些事件作为对田间种植甘蔗温度变化的响应的动态调整。通过对5个甘蔗时钟基因ScLHY、ScPRR37、ScPRR73、scprr95和sctoc1的系统搜索，研究人员在4个月龄植株的冬季和9个月龄植株的夏季取样甘蔗的不同器官，发现所有基因中至少有一个选择性剪接异构体的表达依赖于温度和器官。AS异构体的表达随季节而变化。我们的研究结果表明，生物钟基因中的AS事件受温度调节。
Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to identify AS events in core circadian clock genes and the dynamic adjustment of the events as a response to temperature changes in field-grown sugarcane. A systematic search for AS in five sugarcane clock genes,ScLHY,ScPRR37,ScPRR73, ScPRR95andScTOC1using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are regulated by temperature.