DIFFERENT FORMS OF THE NEUROTROPHIN RECEPTOR TRKB MESSENGER-RNA PREDOMINATE IN RAT RETINA AND OPTIC-NERVE
DIFFERENT FORMS OF THE NEUROTROPHIN RECEPTOR TRKB MESSENGER-RNA PREDOMINATE IN RAT RETINA AND OPTIC-NERVE
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DOI：
10.1002/neu.480240907
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发表时间：
1993-09-01
期刊：
影响因子：
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通讯作者：
AGUAYO, AJ
中科院分区：
文献类型：
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作者：
JELSMA, TN;FRIEDMAN, HH;AGUAYO, AJ
在大鼠视网膜和视神经中对TrkB mRNA的表达进行了研究。在视网膜RNA的Northern印迹中发现编码全长TRKB的11.5kb转录本占主导地位。通过原位杂交，这种TrkB表达集中在神经节细胞层和内核层。此外，一种针对全长TRKB的抗体对视网膜神经节细胞及其轴突进行了免疫染色。相比之下，视神经RNA的Northern印迹显示出一条显著的9.5kb条带，其编码一种缺乏酪氨酸激酶结构域的TRKB受体形式。在坐骨神经以及培养的星形胶质细胞和C6胶质瘤细胞中也检测到了这种形式。这些结果表明，神经元表达含有酪氨酸激酶结构域的全长TRKB，而非神经元细胞表达该受体的截短形式。这两类TRKB可能在视网膜和视神经中介导不同的神经营养作用。（C）1993年约翰威利父子公司
The expression of TrkB mRNAs was investigated in rat retina and optic nerve. A 11.5 kb transcript that encodes full-length TRKB was found to predominate in Northern blots of retinal RNA. By in situ hybridization, this trkB expression was concentrated in the ganglion cell and inner nuclear layers. Furthermore, an antibody to the full-length TRKB immunostained retinal ganglion cells and their axons. In contrast, Northern blots of optic nerve RNA showed a prominent 9.5 kb band that encoded a form of the TRKB receptor lacking the tyrosine kinase domain. This species was also detected in both the sciatic nerve and cultured astrocytes and C6 glioma cells. These results suggest that neurons express the full-length TRKB containing the tyrosine kinase domain, while non-neuronal cells express the truncated form of the receptor. These two classes of TRKB may mediate different neurotrophic actions in the retina and optic nerve. (C) 1993 John Wiley & Sons, Inc.