Simultaneous Spectrophotometric Estimation of Cefepime and Tazobactam in Pharmaceutical Dosage Form
Simultaneous Spectrophotometric Estimation of Cefepime and Tazobactam in Pharmaceutical Dosage Form
复制标题
药物剂型中头孢吡肟和他唑巴坦的同时分光光度法评估
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
S. Patil
中科院分区：
文献类型：
--
作者：
R. Nanda;Dipak A. Navathar;Amol A. Kulkarni;S. Patil
建立了两种同时测定药物制剂中头孢吡肟和他唑巴坦含量的分光光度法。第一种方法采用Vierodt法（联立方程法）进行测定;选择的进样波长为232 nm和262 nm，头孢吡肟和他唑巴坦的浓度范围分别为5-50μg/mL和2.5-17.5 μg/mL。第二种方法是采用多组分模式法进行测定;选择的进样波长为232 nm和262 nm，头孢吡肟和他唑巴坦的浓度范围分别为5-50 μg/mL和2.5 - 17.5 μg/mL。对分析结果进行了统计学验证，并根据ICH指导原则进行了回收率研究。
Two simple, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Cefepime and Tazobactam in pharmaceutical dosage forms. The first method involves determination using the Vierodt’s Method (Simultaneous Equation Method); the sampling wavelengths selected are 232 nm and 262 nm over the concentration ranges of 5-50μg/mL and 2.5-17.5 μg/mL for Cefepime and Tazobactam respectively. The second method involves `determination using the Multicomponent Mode Method; the sampling wavelengths selected are 232 nm and 262 nm over the concentration ranges of 5-50 μg/mL and 2.517.5 μg/mL for Cefepime and Tazobactam respectively. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guidelines.