Herpes simplex virus type 1 recombination: role of DNA replication and viral a sequences.
Herpes simplex virus type 1 recombination: role of DNA replication and viral a sequences.
复制标题
单纯疱疹病毒 1 型重组：DNA 复制和病毒 a 序列的作用。
DOI：
10.1128/jvi.66.1.277-285.1992
复制
发表时间：
1992
影响因子：
5.4
通讯作者：
Lehman,IR
中科院分区：
文献类型：
--
作者：
Dutch,RE;Bruckner,RC;Mocarski,ES;Lehman,IR
在感染过程中，单纯疱疹病毒1型(HSV-1)基因组的元件会发生倒置，这一过程被认为是通过病毒a序列发生的。为了研究这种重组事件的机制，我们开发了一种检测Vero细胞中瞬时保持的质粒中直接重复a序列两侧的DNA片段缺失的方法。通过这项检测，我们观察到在HSV-1感染细胞中复制的质粒中有很高的重组频率(约8%)。我们还发现，导入未感染细胞的质粒中的a序列与HSV-1感染细胞中未复制的质粒中的a序列之间存在低水平的重组。在复制质粒时，序列之间的重组发生的频率是大小相似的不同序列的直接重复拷贝的两倍。序列之间的重组似乎与复制几乎同时发生，这表明复制和重组的过程是紧密相连的。
During the course of infection, elements of the herpes simplex virus type 1 (HSV-1) genome undergo inversion, a process that is believed to occur through the viral a sequences. To investigate the mechanism of this recombinational event, we have developed an assay that detects the deletion of DNA segments flanked by directly repeated a sequences in plasmids transiently maintained in Vero cells. With this assay, we have observed a high frequency of recombination (approximately 8%) in plasmids that undergo replication in HSV-1-infected cells. We also found a low level of recombination between a sequences in plasmids introduced into uninfected cells and in unreplicated plasmids in HSV-1-infected cells. In replicating plasmids, recombination between a sequences occurs at twice the frequency seen with directly repeated copies of a different sequence of similar size. Recombination between a sequences appears to occur at approximately the same time as replication, suggesting that the processes of replication and recombination are closely linked.