Curcumin Disorders 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine Membranes and Favors the Formation of Nonlamellar Structures by 1,2-Dielaidoyl-sn-glycero-3-phosphoethanolamine
Curcumin Disorders 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine Membranes and Favors the Formation of Nonlamellar Structures by 1,2-Dielaidoyl-sn-glycero-3-phosphoethanolamine
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DOI：
10.1021/jp101045p
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发表时间：
2010-08-05
影响因子：
3.3
通讯作者：
Gomez-Fernandez, Juan C.
中科院分区：
文献类型：
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作者：
Perez-Lara, Angel;Ausili, Alessio;Gomez-Fernandez, Juan C.
姜黄素是存在于姜黄中的一种多酚，姜黄是一种广泛用于亚洲传统医药和烹饪的香料。它具有多种多样的生物效应，并被纳入细胞膜。本文描述了姜黄素调节1,2-二棕榈酰- n-甘油-3-磷酸胆碱（DPPC）和1,2-二酰- n-甘油-3-磷酸纳米胺（DEPE）多层膜物理性质的模式。通过DSC， FT-IR, H-2 NMR， WAXD和SAXD观察，姜黄素在温度低于T-c时使DPPC膜紊乱。姜黄素诱导的T-c减少表明其在双分子层中定向，其主轴平行于酰基链。在T-c以上，姜黄素也引入了紊乱，通过红外光谱可以看出，姜黄素也改变了DPPC的极性基团的构象，增加了未水化的C=O基团的百分比，但不与DPPC的C=O基团或磷酸基团形成氢键。小角度x射线衍射显示，由于姜黄素的存在，重复间距显著增加，表明形成了波纹相。姜黄素浓度的增加逐渐改变了相变的开始和完成，δ H也达到了DPPC/姜黄素的6:1摩尔比。姜黄素浓度的进一步增加对过渡参数没有影响，表明姜黄素在DPPC中的溶解度是有限度的。此外，当用DEPE测试姜黄素对磷脂多态性的影响时，发现H-II相形成的温度降低，表明姜黄素有利于膜的负曲率，这可能是解释其对膜动力学和膜蛋白或可通过膜插入激活的蛋白质的影响的重要原因。
Curcumin is a polyphenol present in turmeric, a spice widely used in Asian traditional medicine and cooking. It has many and diverse biological effects and is incorporated in cell membranes. This paper describes the mode in which curcumin modulates the physical properties of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dielaidyl-sn-glycero-3-phosphoetnanolamine (DEPE) multilamellar membranes. Curcumin disordered DPPC membranes at temperatures below T-c as seen by DSC, FT-IR, H-2 NMR, WAXD, and SAXD. The decrease induced by curcumin in T-c suggested that it is oriented in the bilayer with its main axis parallel to the acyl chains. Above T-c, too, curcumin introduced disorder as seen by infrared spectroscopy which showed that curcumin also alters the conformation of the polar group of DPPC, increasing the percentage of unhydrated C=O groups, but does not form hydrogen bonds with either the C=O group or the phosphate group of DPPC. Small angle X-ray diffraction showed a notable increase in the repeating spacings as a result of the presence of curcumin, suggesting the formation of a rippled phase. Increasing concentrations of curcumin progressively modified the onset and completion of the phase transition and also Delta H up to a 6:1 DPPC/curcumin molar ratio. A further increase of curcumin concentration did not produce effects on the transition parameters, suggesting that there is a limit for the solubility of curcumin in DPPC. Additionally, when DEPE was used to test the effect of curcumin on the phospholipid polymorphism, it was found that the temperature at which the H-II phase is formed decreased, indicating that curcumin favors negative curvature of the membrane, which may be important for explaining its effect on membrane dynamics and on membrane proteins or on proteins which may be activated through membrane insertion.