Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression
Isolation of a differentially spliced C-type flower specific AG-like MADS-box gene from Crocus sativus and characterization of its expression
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DOI：
10.1007/s10535-005-0041-6
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发表时间：
2005-12
影响因子：
1.5
通讯作者：
A. Tsaftaris;K. Pasentsis;A. Polidoros
中科院分区：
文献类型：
--
作者：
A. Tsaftaris;K. Pasentsis;A. Polidoros
我们克隆并鉴定了西红花AGAMOUS1(CsAG1)基因的表达，该基因可能是C型MADS-box基因，与三倍体单子叶植物藏红花的AGAMOUS(AG)同源。鉴定了MIKC型植物MADS蛋白的典型结构域结构。氨基酸序列的系统发育分析表明，该基因与风信子和蝴蝶兰的AGAMOU同源基因形成了一个分支。发现了一个差异剪接事件，改变了C末端的氨基酸序列，导致形成了两个大小不同的十个核苷酸的mRNAs。这两个差异剪接转录本的存在仅限于成熟的番红花，特别是雄蕊和心皮。
We have cloned and characterized the expression ofCrocus sativus AGAMOUS1(CsAG1), a putative C-type MADS-box gene homologous toAGAMOUS(AG) from a triploid monocot species crocus (Crocus sativusL.). The typical domain structure of MIKC-type plant MADS proteins was identified. Phylogenetic analysis of the deduced amino acid sequence indicated that the isolated gene forms a clade with theAGAMOUShomologs from the monocotsHyacinthus orientalisandPhalaenopsis equestris. A differential splicing event altering the amino acid sequence at the C terminus was identified, leading to the formation of two mRNAs differing ten nucleotides in size. The presence of both differentially spliced transcripts was restricted only to mature crocus flowers and particularly to stamens and carpels.