Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses
Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses
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DOI：
10.1021/ac701727r
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发表时间：
2008-01-01
影响因子：
7.4
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
--
作者：
Livesay, Eric A.;Tang, Keqi;Smith, Richard D.
我们描述了一个四柱，高压毛细管液相色谱（ILC）系统的鲁棒性，高通量液相色谱-质谱（LC-MS（/MS））分析。该系统并行执行多个LC分离，但交错它们中的每一个，使得每个分离的数据丰富区域被顺序地采样。通过允许几乎连续的数据采集，这种设计最大限度地利用质谱仪。每个分析柱连接到相应的ESI发射器，以避免使用柱后切换和相关的死体积问题。采用编码平移级将发射器顺序定位在MS入口处。该系统的高重现性证明了使用连续分析的全球胰蛋白酶消化的微生物希瓦氏菌oneidensis。
We describe a four-column, high-pressure capillary liquid chromatography (ILC) system for robust, high-throughput liquid chromatography-mass specbrometry (LC-MS(/MS)) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of postcolumn switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.