Effect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats
Effect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats
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DOI：
10.1016/j.ultras.2008.01.009
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发表时间：
2008-09-01
期刊：
影响因子：
4.2
通讯作者：
Szymanski de Toledo, Olga Maria
中科院分区：
文献类型：
--
作者：
Balejo Piedade, Maria Cristina;Galhardo, Milene Sanches;Szymanski de Toledo, Olga Maria
本研究的目的是评估脉冲超声疗法 (PUT) 在 30 只 Wistar 大鼠撕裂性腓肠肌损伤实验模型中刺激肌肉再生和胶原沉积的效果。每天用 1 MHz 脉冲超声 (50%)、0.57 W/cm(2) 治疗 15 只大鼠 (TG) 5 分钟，15 只为对照动物 (CG)。术后第 4、7 和 14 天通过 H&E、Picrosirius 极化和结蛋白免疫组织化学分析肌肉样本。治疗组和对照组的病变均表现出相似的炎症反应。术后 4 天（17.53 +/- 6.2% vs 6.79 +/- 1.3%，p = 0.0491）、7 天（31.07 +/- 7.45% vs 12.57 +/- 3.6%，p = 0.0021）和 14 天（30.39 +/- 0.0021）时，TG 中纤维状胶原的面积分数更大7.3% 对比 19.13 +/- 3.51%，p = 0.0118)；术后 14 天，TG 中成肌细胞和肌管的面积分数更大（41.66 +/- 2.97% vs 34.83 +/- 3.08%，p = 0.025）。我们的数据表明，PUT 增加了肌肉谱系细胞的分化，这将有利于组织再生。另一方面，也表明胶原纤维沉积较多，这可能意味着功能性能较差。然而，在第 7 天，TG 中的纤维百分比似乎已稳定，而 CG 中的纤维百分比则持续增加。此外，根据天狼星红染色结果，TG实现的胶原超分子组织也很重要。 (C) 2008 Elsevier B.V. 保留所有权利。
The aim of this study was to evaluate the effect of the pulsed ultrasound therapy (PUT) in stimulating myoregeneration and collagen deposition in an experimental model of lacerative gastrocnemius muscle lesion in 30 Wistar rats. Fifteen rats were treated (TG) daily with 1 MHz pulsed ultrasound (50%) at 0.57 W/cm(2) for 5 min, and 15 were control animals (CG). Muscle samples were analyzed on postoperative days 4, 7 and 14 through H&E, Picrosirius-polarization and immunohistochemistry for desmin. The lesions presented similar inflammatory responses in both treated and control groups. The areal fraction of fibrillar collagen was larger in the TG at 4 days post-operatively (17.53 +/- 6.2% vs 6.79 +/- 1.3%, p = 0.0491), 7 days (31.07 +/- 7.45% vs 12.57 +/- 3.6%, p = 0.0021) and 14 days (30.39 +/- 7.3% vs 19.13 +/- 3.51%, p = 0.0118); the areal fraction of myoblasts and myotubes was larger in the TG at 14 days after surgery (41.66 +/- 2.97% vs 34.83 +/- 3.08%, p = 0.025). Our data suggest that the PUT increases the differentiation of muscular lineage cells, what would favor tissue regeneration. On the other hand, it is also suggested that there is a larger deposition of collagenous fibers, what could mean worse functional performance. However, the percentage of fibers seems to have stabilized at day 7 in TG and kept increasing in CG. Furthermore, the collagen supramolecular organization achieved by the TG is also significant according to the Sirius red staining results. (C) 2008 Elsevier B.V. All rights reserved.