Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: Comparison with ELISA
Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: Comparison with ELISA
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DOI：
10.1016/j.jim.2009.09.001
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发表时间：
2009-10-31
影响因子：
2.2
通讯作者：
Rinaldi, Sabina
中科院分区：
文献类型：
--
作者：
Dossus, Laure;Becker, Susen;Rinaldi, Sabina
多重免疫分析(MIA)允许在小样本量下同时测量不同的细胞因子，但其在参与大规模流行病学研究的“健康”受试者样本中的适用性尚不为人所知。本文比较了36例健康受试者血清和血浆中白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)、白介素1受体拮抗剂(IL-1ra)、C反应蛋白(CRP)和可溶性CD40L(SCD40L)的检测结果。我们观察到MIA和ELISA法测定IL-1ra、CRP和sCD40L之间具有良好的相关性(r>；0.80)，而IL-6、TNF-α和IL-1β的相关性较差(r<；0.40)。当比较MIA血浆和血清测定时，CRP的相关性很高(r=0.98)，而IL-1ra的相关性较好(r=0.60)。总而言之，在大规模流行病学研究中，基于多重分析的分析可以准确地估计相对风险，但只能对存在相对较大浓度(ng/mL)的细胞因子进行评估。(C)2009爱思唯尔B.V.保留所有权利。
Multiplex-based immunoassays (MIA) allow the simultaneous measurement of different cytokines in small sample Volumes, but their applicability in samples from "healthy" subjects, as those participating in large-scale epidemiological studies is not well known. We compared measurements of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-1 receptor antagonist (IL-1Ra), C-reactive protein (CRP) and soluble CD40L (sCD40L) obtained by MIA with those obtained by enzyme-linked immunosorbent assays (ELISA) in serum and plasma samples from 36 "healthy" subjects. We observed good correlations between measurements obtained by MIA and ELISA for IL-1Ra, CRP and sCD40L (r > 0.80) but poor correlations for IL-6, TNF-alpha and IL-1 beta (r < 0.40). When comparing MIA plasma and serum measurements, very high correlations were obtained for CRP (r = 0.98) and fairly good correlations for IL-1Ra (r = 0.60). In conclusion, multiplex-based assays can give an accurate estimate of the relative risk in large-scale epidemiological studies but only for cytokines that are present in relatively large concentrations (ng/mL). (C) 2009 Elsevier B.V. All rights reserved.