Tissue Engineering with Compact Bone-Derived Cell Spheroids Enables Bone Formation around Transplanted Tooth
Tissue Engineering with Compact Bone-Derived Cell Spheroids Enables Bone Formation around Transplanted Tooth
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DOI：
10.1007/s13770-021-00423-3
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发表时间：
2022-02
影响因子：
3.6
通讯作者：
Nahomi Matsumura;Xianqi Li;Eri Uchikawa-Kitaya;Ni Li;Hongwei Dong;Kai Chen;M. Yoshizawa;H. Kaga
中科院分区：
文献类型：
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作者：
Nahomi Matsumura;Xianqi Li;Eri Uchikawa-Kitaya;Ni Li;Hongwei Dong;Kai Chen;M. Yoshizawa;H. Kaga
虽然牙齿移植是治疗儿童恒牙先天性缺损的理想选择，但移植到狭窄的牙槽嵴是不可行的。在这项研究中，我们探讨了骨组织工程的可能性，同时与牙齿移植，以增加牙槽骨的宽度。方法骨髓单个核细胞或皮质骨来源的间充质基质细胞球体接种到去端胶原海绵和移植新鲜提取的磨牙从同一品系的小鼠。使用显微计算机断层扫描和组织学分析评估牙根周围的新骨形成。单独的牙齿，或牙齿与支架，但没有细胞，也被移植，并作为对照组。在皮质骨源性间充质基质细胞组中也观察到明显的根外骨形成，但在其他三组中很少。组织学分析显示，新骨和根之间的空间充满了胶原纤维，在所有四个组，表明牙周膜maintained.CONCLUSIONThis研究表明，潜在的同时牙槽骨扩张采用骨组织工程的方法，使用皮质骨来源的间充质基质细胞球体的牙齿移植。原位移植模型的使用可以进一步阐明移植牙齿在较长时间内的可行性和功能恢复。
BACKGROUNDAlthough tooth transplantation is a desirable treatment option for congenital defects of permanent teeth in children, transplantation to a narrow alveolar ridge is not feasible. In this study, we investigated the possibility of bone tissue engineering simultaneously with tooth transplantation to enhance the width of the alveolar bone.METHODSBone marrow mononuclear cells or cortical bone-derived mesenchymal stromal cell spheroids were seeded onto atelocollagen sponge and transplanted with freshly extracted molars from mice of the same strain. New bone formation around the tooth root was evaluated using micro-computed tomography and histological analysis. Tooth alone, or tooth with scaffold but without cells, was also transplanted and served as controls.RESULTSMicro-computed tomography showed new bone formation in the furcation area in all four groups. Remarkable bone formation outside the root was also observed in the cortical bone-derived mesenchymal stromal cell group, but was scarce in the other three groups. Histological analysis revealed that the space between the new bone and the root was filled with collagen fibers in all four groups, indicating that the periodontal ligament was maintained.CONCLUSIONThis study demonstrates the potential of simultaneous alveolar bone expansion employing bone tissue engineering approach using cortical bone-derived mesenchymal stromal cell spheroids for tooth transplantation. The use of an orthotopic transplantation model may further clarify the feasibility and functional recovery of the transplanted tooth over a longer period.