Metabolome analysis of photosynthesis and the related primary metabolites in the leaves of transgenic rice plants with increased or decreased Rubisco content.
Metabolome analysis of photosynthesis and the related primary metabolites in the leaves of transgenic rice plants with increased or decreased Rubisco content.
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DOI：
10.1111/j.1365-3040.2012.02494.x
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Yuji Suzuki;Tamaki Fujimori;K. Kanno;A. Sasaki;Yoshiaki Ohashi;A. Makino
中科院分区：
文献类型：
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作者：
Yuji Suzuki;Tamaki Fujimori;K. Kanno;A. Sasaki;Yoshiaki Ohashi;A. Makino
由于叶片Rubisco含量的遗传操作对代谢的综合影响是未知的，利用毛细管电泳-飞行时间质谱（CE-TOFMS）技术对Rubisco含量增加或减少的转基因水稻植株进行代谢组学分析。在RBCS感植物中，Rubisco含量的增加并没有改善光饱和光合作用。甘油醛3-磷酸和景天庚酮糖7-磷酸水平增加，但核酮糖二磷酸（RuBP），ATP和ADP水平不受影响。从这些结果可以认为，不依赖于ATP供应的RuBP再生成为光合作用的瓶颈。在RBCS反义植物中，Rubisco含量的下降降低了光合作用，RuBP大量积累。ATP和ADP水平也增加，并与其他核苷的二磷酸和三磷酸化合物的增加有关。这些结果意味着Rubisco含量的下降减缓了卡尔文循环，并且由此产生的ATP的多余能量被转移到其他核苷二磷酸和三磷酸。红细胞计数反义植物的氨基酸水平有下降的趋势，而红细胞计数反义植物的氨基酸水平有上升的趋势，这可能反映了Rubisco合成的需要。淀粉和碳水化合物水平下降，只有在RBCS反义植物。因此，Rubisco含量的遗传操作广泛影响水稻的C和N代谢。
Because the comprehensive effects on metabolism by genetic manipulation of leaf Rubisco content are unknown, metabolome analysis was carried out on transgenic rice plants with increased or decreased Rubisco content using the capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) technique. In RBCS-sense plants, an increase in Rubisco content did not improve light-saturated photosynthesis. Glyceraldehyde 3-phosphate and sedoheputulose 7-phosphate levels increased, but ribulose bisphosphate (RuBP), ATP and ADP levels were not affected. It is considered from these results that RuBP regeneration independent of ATP supply became a bottleneck for photosynthesis. In RBCS-antisense plants, a decline in Rubisco content decreased photosynthesis with a substantial accumulation of RuBP. ATP and ADP levels also increased and were associated with increases in the diphosphate and triphosphate compounds of other nucleosides. These results imply that a decline in Rubisco content slowed down the Calvin cycle and that the resultant excess energy of ATP was transferred to other nucleoside diphosphates and triphosphates. The levels of amino acids tended to decline in RBCS-sense plants and increase in RBCS-antisense plants, probably reflecting the demand for Rubisco synthesis. Starch and carbohydrate levels decreased only in RBCS-antisense plants. Thus, genetic manipulation of Rubisco contents widely affected C and N metabolism in rice.