Identification of protective Lassa virus epitopes that are restricted by HLA-A2
Identification of protective Lassa virus epitopes that are restricted by HLA-A2
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DOI：
10.1128/jvi.00896-06
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发表时间：
2006-09-01
影响因子：
5.4
通讯作者：
Buchmeier, Michael J.
中科院分区：
文献类型：
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作者：
Botten, Jason;Alexander, Jeff;Buchmeier, Michael J.
从拉沙病毒（LASV）感染中恢复通常先于中和抗体的出现，表明细胞免疫在病毒清除中起主要作用。迄今为止，LASV特异性CD 8（+）T细胞的作用尚未在人体中进行评估。为了促进这些研究，我们利用预测算法从LASV核蛋白和糖蛋白前体（GPC）基因中鉴定候选HLA-A2超型表位。我们鉴定了三种肽（GPC（42-50），GLVGLVTFL; GPC（60-68），SLYKGVYEL;和GPC（441-449），YLISIFLHL），其显示高亲和力结合（
Recovery from Lassa virus (LASV) infection usually precedes the appearance of neutralizing antibodies, indicating that cellular immunity plays a primary role in viral clearance. To date, the role of LASV-specific CD8(+) T cells has not been evaluated in humans. To facilitate such studies, we utilized a predictive algorithm to identify candidate HLA-A2 supertype epitopes from the LASV nucleoprotein and glycoprotein precursor (GPC) genes. We identified three peptides (GPC(42-50), GLVGLVTFL; GPC(60-68), SLYKGVYEL; and GPC(441-449), YLISIFLHL) that displayed high-affinity binding (