A SIMPLIFIED ULTRASENSITIVE SILVER STAIN FOR DETECTING PROTEINS IN POLYACRYLAMIDE GELS
A SIMPLIFIED ULTRASENSITIVE SILVER STAIN FOR DETECTING PROTEINS IN POLYACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(80)90470-4
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
MORRIS, NR
中科院分区：
文献类型：
--
作者：
OAKLEY, BR;KIRSCH, DR;MORRIS, NR
采用高灵敏度的银染法对红曲霉进行染色。C.瑞士三角R. Merril和S.简化了Shifrin用于聚丙烯酰胺凝胶中蛋白质的可视化。手术步骤从10步减少到6步，简化了每一步中的试剂，所需的银量减少了10倍，大大降低了该过程的费用。与最初的银染色相同，该方法的灵敏度比考马斯亮蓝高100倍，并且与放射性标记蛋白质的放射自显影的灵敏度相当。
The highly sensitive silver stain of R. C. Switzer III, C. R. Merril and S. Shifrin for visualizing proteins in polyacrylamide gels was simplified. The number of steps in the procedure was reduced from 10 to 6., the reagents in each step were simplified and the amount of silver required was reduced by a factor of 10, greatly reducing the expense of the procedure. In common with the original silver stain, this procedure is 100 times more sensitive than Coomassie brilliant blue and is comparable in sensitivity to autoradiography of radioactively labeled proteins.