Transcriptional response of Enterococcus faecalis V583 to erythromycin
Transcriptional response of Enterococcus faecalis V583 to erythromycin
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DOI：
10.1128/aac.49.6.2246-2259.2005
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发表时间：
2005-06-01
影响因子：
4.9
通讯作者：
Nes, IF
中科院分区：
文献类型：
--
作者：
Aakra, Å;Vebo, H;Nes, IF
展示了用红霉素处理的粪肠球菌 V583 (V583) 的转录谱。这是第一项描述肠球菌完整转录谱的研究。粪肠球菌是许多自然环境中常见的无毒力细菌，也是医院感染的重要原因。我们使用基于 V583 基因组序列的全基因组微阵列来研究暴露于红霉素的细胞中的基因表达。 V583 对相对高浓度的红霉素具有抗性，但治疗会延缓生长。通过时程实验研究红霉素处理对V583的影响；在 90 分钟内的五个时间点提取样品。与未处理的细胞相比，红霉素处理的细胞的基因转录发生了巨大的变化。总共有 260 个基因在一个或多个时间点下调，而 340 个基因上调。编码假设蛋白的基因和编码转运蛋白和结合蛋白的基因是差异表达基因的两个最主要的组。编码ermB (EFA0007)的基因被表达，但没有差异，这表明其他基因对于红霉素处理的V583的存活和生长维持很重要。其中一个基因是一种假定的 MsrC 样蛋白，该蛋白在研究的所有时间点均上调。其他被发现上调的特定基因是编码 ABC 转运蛋白和二元调控系统的基因，这些基因可能对 V583 对红霉素的特异性反应很重要。
A transcriptional profile of Enterococcus faecalis V583 (V583) treated with erythromycin is presented. This is the first study describing a complete transcriptional profile of Enterococcus. E. faecalis is a common and nonvirulent bacterium in many natural environments, but also an important cause of nosocomial infections. We have used a genome-wide microarray based on the genome sequence of V583 to study gene expression in cells exposed to erythromycin. V583 is resistant to relatively high concentrations of erythromycin, but growth is retarded by the treatment. The effect of erythromycin treatment on V583 was studied by a time course experiment; samples were extracted at five time points over a period of 90 min. A drastic change in gene transcription was seen with the erythromycin-treated cells compared to the untreated cells. Altogether, 260 genes were down-regulated at one or more time points, while 340 genes were up-regulated. Genes encoding hypothetical proteins and genes encoding transport and binding proteins were the two most dominating groups of differentially expressed genes. The gene encoding ermB (EFA0007) was expressed, but not differentially, which indicated that other genes are important for the survival and growth maintenance of V583 treated with erythromycin. One of these genes is a putative MsrC-like protein, which was up-regulated at all time points studied. Other specific genes that were found to be up-regulated were genes encoding ABC transporters and two-component regulatory systems, and these may be genes that are important for the specific response of V583 to erythromycin.