An artificial extracellular matrix created by hepatocyte growth factor fused to IgG-Fc
An artificial extracellular matrix created by hepatocyte growth factor fused to IgG-Fc
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DOI：
10.1016/j.biomaterials.2009.09.105
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发表时间：
2010-02-01
期刊：
影响因子：
14
通讯作者：
Akaike, Toshihiro
中科院分区：
文献类型：
--
作者：
Azuma, Koji;Nagaoka, Masato;Akaike, Toshihiro
人工细胞外基质（ECM）的设计在组织工程、再生医学和分子生物学研究中备受关注。构建了融合免疫球蛋白G (IgG) Fc区（简称AeHGF-Fc）的重组肝细胞生长因子（HGF），并通过Western blot检测证实。aehgf - fc包被表面的HepG2细胞粘附量与胶原包被表面的HepG2细胞粘附量几乎相同，但形态变化较大。在HepG2细胞中，固定化的AeHGF-Fc持续激活Akt，而可溶性HGF诱导的Akt激活随着时间的推移而迅速降低，表明与可溶性HGF相比，固定化的AeHGF-Fc遵循不同的信号转导途径。(C) 2009年Elsevier Ltd.出版
The design of artificial extracellular matrices (ECM) has attracted much attention in tissue engineering and regenerative medicine as well as in molecular biology research. A recombinant hepatocyte growth factor (HGF), fused to an immunoglobulin G (IgG) Fc region (abbreviated as AeHGF-Fc) was constructed and confirmed by Western blot assay. Almost similar amounts of HepG2 cells adhered to AeHGF-Fc-coated surface compared to collagen-coated one with large morphological changes. Immobilized AeHGF-Fc continuously activated Akt in HepG2 cells whereas Akt activation induced by soluble HGF rapidly decreased with time, indicating that immobilized AeHGF-Fc follows different signal transduction pathways compared to soluble HGF. (C) 2009 Published by Elsevier Ltd.