Identification of bone morphogenetic proteins and their receptors in human breast cancer cell lines: Importance of BMP2
Identification of bone morphogenetic proteins and their receptors in human breast cancer cell lines: Importance of BMP2
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DOI：
10.1006/cyto.1999.0508
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发表时间：
1999-12-01
期刊：
影响因子：
3.8
通讯作者：
McGrath, BE
中科院分区：
文献类型：
--
作者：
Arnold, SF;Tims, E;McGrath, BE
乳腺癌最常见的转移部位是骨，这表明一些乳腺癌表达的蛋白质促进了这一过程。我们评估了高转移性乳腺癌细胞系MDA- mb -231和低转移性乳腺癌细胞系MCF-7是否含有骨形态发生蛋白（BMP），半定量逆转录聚合酶链反应（RT-PCR）表明MDA和MCF-7细胞含有BMP受体IA， IB和II的mrna。RT-PCR显示乳腺细胞中存在BMPs 2和3的mrna，但不存在BMPs 4和7的mrna。使用带有分子信标的RT-PCR策略，我们发现在亚致死剂量的辐射后，MDA细胞中BMP2的mRNA减少了75%。使用BMP2特异性抗体的酶联免疫吸附试验表明，MDA细胞辐照后BMP2蛋白减少。BMP2 mRNA在MCF-7细胞中的表达程度低于MDA细胞，并且在MCF-7细胞放射治疗后没有改变，分子信标RT-PCR证实，重组人BMP2对MDA细胞的增殖抑制程度高于MCF-7细胞。这些结果扩大了含有bmp的组织的数量，并证明了这种信号通路对这些组织生长状态的影响。(C) 1999学术出版社。
The most frequent site of breast cancer metastasis is bone suggesting that some breast cancers express proteins that facilitate this process. We evaluated whether a highly metastatic breast cancer cell line, MDA-MB-231, and a less metastatic breast cancer cell line, MCF-7, contain bone morphogenetic proteins (BMP), Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that MDA and MCF-7 cells contain mRNAs for BMP receptors IA, IB and II. RT-PCR indicated the presence of mRNAs for BMPs 2 and 3 but not 4 and 7 in breast cells. Using a RT-PCR strategy with molecular beacons, we found that the mRNA for BMP2 in MDA cells was decreased by 75% after a sublethal dose of radiation. An ELISA using an antibody specific for BMP2 demonstrated that BMP2 protein was reduced after radiation of MDA cells. The mRNA for BMP2 was expressed to a lesser extent in MCF-7 cells than MDA cells and was not altered after radiation treatment of MCF-7 cells as demonstrated by molecular beacon RT-PCR, Recombinant human BMP2 decreased the proliferation of MDA cells to a greater extent than MCF-7 cells. These results expand the number of tissues that contain BMPs and demonstrate the effect of this signalling pathway of the growth state of these tissues. (C) 1999 Academic Press.