Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer
Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer
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DOI：
10.1021/ac0521955
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发表时间：
2006-07-01
影响因子：
7.4
通讯作者：
Leclerc, Mario
中科院分区：
文献类型：
--
作者：
Le Floch, Fabien;Ho, Hoang A.;Leclerc, Mario
描述了两种使用水溶性二茂铁功能化聚噻吩传感器和单链寡核苷酸适体探针检测人α-凝血酶的无标记电化学方法。第一种方法是直接方法，其中记录的电流在添加目标蛋白质后减少。第二种需要更多的步骤以及额外使用 PNA 探针和核酸酶。这种间接方法导致电信号随着人类 α-凝血酶浓度的变化而增加，检测限为 75 fmol。
Two label-free electrochemical methods for the detection of human alpha-thrombin using a water-soluble, ferrocene-functionalized polythiophene transducer and a single-stranded oligonucleotide aptamer probe are described. The first approach is a direct method in which the recorded current decreases upon addition of the targeted protein. The second one requires more steps and the additional utilization of PNA probes and nuclease enzyme. This indirect method leads to an increase of the electrical signal as a function of the concentration of human alpha-thrombin with a detection limit of 75 fmol.