Dysregulation of GdA Expression in Endometrium of Women With Endometriosis: Implication for Endometrial Receptivity
Dysregulation of GdA Expression in Endometrium of Women With Endometriosis: Implication for Endometrial Receptivity
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DOI：
10.1177/1933719117718276
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发表时间：
2018-04-01
影响因子：
2.9
通讯作者：
Piomboni, Paola
中科院分区：
文献类型：
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作者：
Focarelli, Riccardo;Luddi, Alice;Piomboni, Paola
糖蛋白A(Glycodelin-A，GDA)已被认为是子宫内膜功能的潜在生物标志物，但对其在月经周期不同阶段和病理条件下的表达知之甚少。鉴于GDA在胚胎着床中的潜在重要性，我们的目的是评估GDA在月经周期不同时间的子宫内膜组织中的表达情况以及不同糖化形式的存在和免疫定位。我们的结果表明，GDA是由子宫内膜上皮细胞和间质细胞合成的，在正常子宫内膜和子宫内膜异位症患者的在位内膜中都有合成，其分布包括几种糖基化形式，在月经周期的各个阶段都有不同的表达。在分泌期，子宫内膜异位内膜中GDA蛋白的表达显著增加，并呈现不同的糖形式。在位子宫内膜组织中的蛋白质定位结果与已证实有生育能力的妇女的子宫内膜相比有显著差异。这项研究表明，GDA是一种复杂的糖蛋白，包括多达6种不同的糖形式，在月经周期的不同阶段特异表达；在子宫内膜异位症等病理情况下，表达谱发生改变，可能与子宫内膜容受性受损有关。
Glycodelin-A (GdA) has been proposed to represent a potential biomarker of endometrial function, but little is known about its expression during the different phases of the menstrual cycle and under pathological conditions. In the light of its potential importance also in embryo implantation, we aimed to evaluate the expression profile of GdA as well as the presence of different glycosylated glycoforms and the immunolocalization in endometrial tissue from women with endometriosis and in women with proven fertility, at different times during the menstrual cycle. Our results showed that GdA is synthesized by endometrial epithelial and stromal cells, both in healthy endometrium and eutopic endometrium from women with endometriosis, with a profile including several glycosylated glycoforms, differentially expressed in each phase of the menstrual cycle. During the secretory phase, a significant increase in GdA protein expression, with a different glycoforms profile, was observed in endometriotic eutopic endometrium. Protein localization in eutopic endometrial tissue resulted significantly different in comparison with endometrium from women with proven fertility. This study indicate that GdA is a complex glycoprotein including up to 6 different glycoforms specifically expressed during the different phase of the menstrual cycle; in pathologic conditions such as endometriosis, the expression profile is altered possibly related to the impaired endometrial receptivity.